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| Status |
Public on Jul 01, 2013 |
| Title |
Functional genomic analyses of Bifidobacterium bifidum PRL2010 highlight how this microorganism interact with the human host |
| Platform organism |
Bifidobacterium bifidum PRL2010 |
| Sample organism |
Bifidobacterium bifidum |
| Experiment type |
Expression profiling by array
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| Summary |
Bifidobacteria represents one of the dominant group of microorganisms colonizing the intestine of infants. However, the genetic determinants supporting the establishment and the interaction with the human hosts are still largely unknown. Most commensal bacteria interacting with eukaryotic hosts express adhesive molecules on their surfaces that modulate interaction with host cell receptors or with soluble macromolecules. Whole genome transcription profiling of B. bifidum PRL2010, a strain isolated from infant stool, under in vitro as well as in vivo conditions revealed the expression of few common extracellular proteins among which type 1 pili encoding genes.
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| Overall design |
To investigate the molecular mechanisms sustaining the interaction of PRL2010 strain with the human gut, we first explored the global genome transcription profiling of this strain in a in vitro human model such as in the presence of HT29 cell lines. The transcriptome was analyzed using a custom B. bifidum PRL2010 array representing the 90% of this organism’s protein coding genes. To better evaluate the conserved responses by B. bifidum, the in vivo transcriptomes were quantified against a diverse set of transcriptome patterns identified for in vitro laboratory cultures of the strain, i.e., B. bifidum responses after growth on an cell’s monolayers growth medium (DMEM); B.bifidum responses after growth on synthetic medium (MRS). Briefly, we analized five conditions, two of which are also used as references. Every experiment was performed in duplicate and in vivo condition was performed in quadruplicate.
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| Contributor(s) |
Ventura M |
| Citation(s) |
23776216 |
| Submission date |
Mar 12, 2012 |
| Last update date |
Jul 01, 2013 |
| Contact name |
Marco Ventura |
| E-mail(s) |
marco.ventura@unipr.it
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| Phone |
+390521905666
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| Organization name |
University of Parma
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| Department |
Dept. Life Sciences
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| Lab |
Probiogenomics Lab.
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| Street address |
Viale delle Scienze 11/A
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| City |
Parma |
| ZIP/Postal code |
43124 |
| Country |
Italy |
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| Platforms (1) |
| GPL13951 |
Bifidobacterium bifidum PRL2010 array |
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| Samples (12)
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| GSM893552 |
B. bifidum PRL2010 growth in vivo condition_1 |
| GSM893553 |
B. bifidum PRL2010 growth in vivo condition_2 |
| GSM893554 |
B. bifidum PRL2010 growth in vivo condition_3 |
| GSM893555 |
B. bifidum PRL2010 growth in vivo condition_4 |
| GSM893556 |
B. bifidum PRL2010 in contact with HT29_1 |
| GSM893557 |
B. bifidum PRL2010 in contact with HT29_2 |
| GSM893558 |
B. bifidum PRL2010 growth in DMEM_1 |
| GSM893559 |
B. bifidum PRL2010 growth in DMEM_2 |
| GSM893560 |
B. bifidum PRL2010 growth in mouse feed_1 |
| GSM893561 |
B. bifidum PRL2010 growth in mouse feed_2 |
| GSM893562 |
B. bifidum PRL2010 growth in MRS_1 |
| GSM893563 |
B. bifidum PRL2010 growth in MRS_2 |
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| Relations |
| BioProject |
PRJNA153187 |
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