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| Status |
Public on Oct 14, 2012 |
| Title |
Transcriptional profiling of somatic cells differentiation throughout oocyte competence acquisition in the xenopus ovarian follicles. |
| Organism |
Xenopus laevis |
| Experiment type |
Expression profiling by array
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| Summary |
Somatic cells surrounding the oocyte were sampled at the following stages: developmentally incompetent or poorly competent prophase I oocytes (NC1 oocytes), developmentally competent prophase I oocytes (C1 oocytes), and developmentally competent metaphase II oocytes (C2 oocytes).
NC1 samples were collected from immature stage IV follicles, C1 samples from immature stage VI follicles, and C2 samples from in vitro matured stage VI follicles.
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| Overall design |
Global transcriptional profiling was performed using somatic cells collected from xenopus ovarian follicles during in vivo oocyte developmental competence acquisition. Somatic cells were collected at 3 stages of oogenesis: early stage follicles (stage IV, vitellogenic, prophase I arrested oocytes, meiotically competent but developmentally incompetent, n=5), late stage follicles (stage VI, post-vitellogenic, prophase I arrested oocytes, meiotically competent and developmentally competent, n=5) and ovulatory follicles collected after in vitro maturation induction with hCG of post-vitellogenic follicles (metaphase II arrested oocytes, developmentally fully competent, n=5).
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| Contributor(s) |
Charlier C, Uzbekova S, Bobe J, Chesnel F |
| Citation(s) |
23083410 |
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| Submission date |
Mar 19, 2012 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Julien Bobe |
| E-mail(s) |
Julien.Bobe@rennes.inra.fr
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| Phone |
(33) 2 23 48 57 24
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| Organization name |
Institut National de la Recherche Agronomique
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| Lab |
INRA-SCRIBE
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| Street address |
Campus de Beaulieu
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| City |
Rennes |
| ZIP/Postal code |
35000 |
| Country |
France |
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| Platforms (1) |
| GPL11258 |
Agilent-015066 Xenopus Gene Expression Microarray (Probe Name version) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE36617 |
Contribution of molecular actors from somatic origin to oocyte developmental competence acquisition, lessons from evolution |
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| Relations |
| BioProject |
PRJNA155645 |
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