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| Status |
Public on Oct 14, 2012 |
| Title |
Transcriptional profiling of cumulus cells differentiation throughout oocyte competence acquisition in the murine ovarian follicles. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Cumulus cells surrounding the oocyte were sampled at the following stages: developmentally incompetent or poorly competent prophase I oocytes (NC1 oocytes), developmentally competent prophase I oocytes (C1 oocytes), and developmentally competent metaphase II oocytes (C2 oocytes).
NC1 samples were collected from immature, unexpanded cumulus-oocytes complexes (COC) from prepubertal (3-week-old) mice, C1 samples from immature, unexpanded cumulus-oocytes complexes (COC) from adult (8-week-old) and C2 samples from mature, expanded COCs obtained from the oviduct from 8-week-old mice after standard superovulation protocol.
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| Overall design |
Global transcriptional profiling was performed using cumulus cells collected from murine ovarian follicles during in vivo oocyte developmental competence acquisition. Cumulus cells were collected at 3 stages: early stage follicles (prophase I arrested oocytes, meiotically competent but developmentally incompetent, n=5), late stage follicles (prophase I arrested oocytes, meiotically competent and developmentally competent, n=5) and ovulatory follicles collected in vivo (metaphase II arrested oocytes, developmentally fully competent, n=5).
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| Contributor(s) |
Charlier C, Uzbekova S, Bobe J, Chesnel F |
| Citation(s) |
23083410 |
| |
| Submission date |
Mar 19, 2012 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Julien Bobe |
| E-mail(s) |
Julien.Bobe@rennes.inra.fr
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| Phone |
(33) 2 23 48 57 24
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| Organization name |
Institut National de la Recherche Agronomique
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| Lab |
INRA-SCRIBE
|
| Street address |
Campus de Beaulieu
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| City |
Rennes |
| ZIP/Postal code |
35000 |
| Country |
France |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE36617 |
Contribution of molecular actors from somatic origin to oocyte developmental competence acquisition, lessons from evolution |
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| Relations |
| BioProject |
PRJNA155647 |
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