|
| Status |
Public on Mar 28, 2012 |
| Title |
sphingo-1-Identification of Sphingolipid regulated genes in cold stress response. Sphingolipids regulated genes. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
rs07-05_sphingolipids-cold - sphingo-1 - The cold choc response seems to be partly triggered by Sphingolipid species. To date no gene response as been associated to sphingolipid signaling pathway in plant. Our aim is to identify among the cold induced genes the ones regulated by sphingolipids and to try to define a sphingolipid pathway specific group of genes. - 7ml of 5 days-old cells suspensions were incubated in presence of different sphingolipid pathway inhibitors, 30 min to 2 hours depending in the coumpound (all were resuspended in DMSO and control were done with DMSO). Then a 30 min cold choc was applied before cells were harvested and frozen in cold nitrogen. RNA were then extracted. FB1 and DMS were from Alexis , Myr from Cayman, TSP from matreya.
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| |
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| Overall design |
6 dye-swap - treated vs untreated comparison
|
| Web link |
http://urgv.evry.inra.fr/cgi-bin/projects/CATdb/consult_expce.pl?experiment_id=192
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| |
|
| Contributor(s) |
Guillas I, Taconnat L, Renou J, Balzergue S, Martin-Magniette M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 21, 2012 |
| Last update date |
May 03, 2012 |
| Contact name |
Soubigou-Taconnat Ludivine |
| E-mail(s) |
soubigou@evry.inra.fr
|
| Organization name |
INRA
|
| Department |
URGV
|
| Lab |
ADT
|
| Street address |
2 rue gaston crémieux
|
| City |
evry |
| ZIP/Postal code |
91000 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL4346 |
URGV Arabidopsis thaliana 25K CATMA_v2.2 |
|
| Samples (12)
|
| GSM898202 |
13688165 - ctrl 4 degree Celsius 2h vs Fb1 4 degree Celsius 2h |
| GSM898203 |
13688504 - Fb1 4 degree Celsius 2h vs ctrl 4 degree Celsius 2h |
| GSM898204 |
13688169 - ctrl 4 degree Celsius 2h vs Myr 4 degree Celsius 2h |
| GSM898205 |
13688505 - Myr 4 degree Celsius 2h vs ctrl 4 degree Celsius 2h |
| GSM898206 |
13688508 - ctrl 4 degree Celsius 30min vs DMS 4 degree Celsius 30min |
| GSM898207 |
13688499 - DMS 4 degree Celsius 30min vs ctrl 4 degree Celsius 30min |
| GSM898208 |
13688507 - ctrl 4 degree Celsius 30min vs TSP 4 degree Celsius 30min |
| GSM898209 |
13688502 - TSP 4 degree Celsius 30min vs ctrl 4 degree Celsius 30min |
| GSM898210 |
13688503 - ctrl 22 degree Celsius 30min vs ctrl 4 degree Celsius 30min |
| GSM898211 |
13688506 - ctrl 4 degree Celsius 30min vs ctrl 22 degree Celsius 30min |
| GSM898212 |
13688509 - ctrl 22 degree Celsius 2h vs ctrl 4 degree Celsius 2h |
| GSM898213 |
13688511 - ctrl 4 degree Celsius 2h vs ctrl 22 degree Celsius 2h |
|
| Relations |
| BioProject |
PRJNA156999 |
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