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| Status |
Public on May 01, 2012 |
| Title |
GY118F downstream effect in EpiSCs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
This microarray was performed to gain insight in the effect of GY118F stimulation in EpiSCs. This array is part of the following paper to be published in Nature Communications: “JAK/STAT3 signalling is sufficient and dominant over antagonistic cues for the establishment of naïve pluripotency” by Anouk L. van Oosten, Yael Costa, Austin Smith & José C.R. Silva
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| Overall design |
Gy0 a, gy0 b, gy0 c (triplicate samples for GY118F EpiSCs in N2B27+Activin+Fgf) c0 a, c0 b, c0 c (triplicate samples for Control EpiSCs in N2B27+Activin+Fgf) gy3 b, gy3 c (duplicate samples for GY118F EpiSCs in N2B27+Activin+Fgf stimulated with GCSF for 3 hours) c3 a, c3 b, c3 c (triplicate samples for Control EpiSCs in N2B27+Activin+Fgf stimulated with GCSF for 3 hours) Within the manucript genes of interest were defined as those that were significantly upregulated (P<0.01) above a 1.4 fold change threshold in GCSF stimulated GY118F EpiSCs as compared to those not stimulated, but for which the expression did not significantly change upon GCSF stimulation in the control. The fold change in expression was calculated by 2^absolute value of subtracted normalised data.
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| Citation(s) |
22569365 |
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| Submission date |
Mar 26, 2012 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Jose Silva |
| Organization name |
University of Cambridge, centre for stem cell research
|
| Street address |
Tennis Court Road
|
| City |
Cambridge |
| ZIP/Postal code |
cb2 1qr |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (11)
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| GSM902364 |
GY118F EpiSCs in N2B27 + Activin + Fgf replicate nr. 1 |
| GSM902365 |
GY118F EpiSCs in N2B27 + Activin + Fgf replicate nr. 2 |
| GSM902366 |
GY118F EpiSCs in N2B27 + Activin + Fgf replicate nr. 3 |
| GSM902367 |
Control EpiSCs in N2B27 + Activin + Fgf replicate nr. 1 |
| GSM902368 |
Control EpiSCs in N2B27 + Activin + Fgf replicate nr. 2 |
| GSM902369 |
Control EpiSCs in N2B27 + Activin + Fgf replicate nr. 3 |
| GSM902370 |
GY118F EpiSCs in N2B27 + Activin + Fgf stimulated with GCSF for 3 hours replicate 1 |
| GSM902371 |
GY118F EpiSCs in N2B27 + Activin + Fgf stimulated with GCSF for 3 hours replicate 2 |
| GSM902372 |
control EpiSCs in N2B27 + Activin + Fgf stimulated with GCSF for 3 hours replicate 1 |
| GSM902373 |
control EpiSCs in N2B27 + Activin + Fgf stimulated with GCSF for 3 hours replicate 2 |
| GSM902374 |
control EpiSCs in N2B27 + Activin + Fgf stimulated with GCSF for 3 hours replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE36818 |
GY118F downstream targets in iPSCs and EpiSCs |
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| Relations |
| BioProject |
PRJNA156855 |
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