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| Status |
Public on Jan 02, 2013 |
| Title |
miRNA expression profile in the Squalius alburnoides complex |
| Platform organisms |
Caenorhabditis elegans; Drosophila melanogaster; Danio rerio; Gallus gallus; Homo sapiens; Mus musculus; Rattus norvegicus; Takifugu rubripes |
| Sample organisms |
Squalius alburnoides; Squalius pyrenaicus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
The Squalius alburnoides complex (Steindachner) is one of the most intricate hybrid polyploid systems known in vertebrates. In this complex, the constant switch of the genome composition in consecutive generations, very frequently involving a change on the ploidy level, promotes repetitive situations of potential genomic shock. Previously in this complex, it was shown that in response to the increase in the genome dosage, triploid hybrids could regulate gene expression to a diploid state. In this work, we compared the small RNA profiles in the different genomic compositions interacting in the complex. Using high-throughput arrays and sequencing technologies, we were able to verify that diploid and triploid hybrids were closely related: they shared most of their sequences and their miRNA expression profiles were highly correlated. However, an overall view indicates an up-regulation of a substantial number of miRNAs in triploids. Also, the global miRNA expression in triploids was higher than predicted from an additive model. These results point to a participation of miRNAs in the cellular functional stability needed when the ploidy change.
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| Overall design |
In this study, miRNA expression from 3 adult tissues (brain, muscle and liver) from 3 individuals of each genomic composition (PAA, PA, AA and PP) was analysed using microarrays.
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| Contributor(s) |
Inácio A, Pinho J, Pereira PM, Comai L, Coelho MM |
| Citation(s) |
22815952 |
| |
| Submission date |
Mar 28, 2012 |
| Last update date |
Jan 02, 2013 |
| Contact name |
Angela Inácio |
| E-mail(s) |
mainacio@fc.ul.pt
|
| Organization name |
centro de Biologia Ambiental
|
| Street address |
Campo Grande
|
| City |
Lisbon |
| ZIP/Postal code |
1749-16 |
| Country |
Portugal |
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| Platforms (1) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE38799 |
miRNA expression profile and small RNA transcriptome in the Squalius alburnoides complex |
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| Relations |
| BioProject |
PRJNA157019 |
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