|
| Status |
Public on Jul 19, 2012 |
| Title |
Analysis of gene expression in differentiated human primary keratinocytes depleted for Grainyhead like 3 (GRHL3) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Human primary keratinocytes were depleted of GRHL3 by siRNA and induced to differentiated for 2 days by addition of Calcium
|
| |
|
| Overall design |
Primary normal human keratinocytes were transfected with GRHL3 or scrambled control siRNA using RNAi max (Life Technologies). 24 hours post transfection medium was raised to 1.8mM to induce differentiation. Cells were collected 48 hours later.
|
| |
|
| Contributor(s) |
Hopkin AS, Andersen B |
| Citation(s) |
22829784 |
| Submission date |
Apr 04, 2012 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Amelia Soto Hopkin |
| E-mail(s) |
sotoa@uci.edu
|
| Phone |
9498249372
|
| Organization name |
University of California Irvine
|
| Department |
Biological Chemistry & Medicine
|
| Lab |
Bogi Andersen
|
| Street address |
250 Sprague Hall
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92617 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (6)
|
| GSM909315 |
scrambled siRNA biological replicate 1 |
| GSM909316 |
scrambled siRNA biological replicate 2 |
| GSM909317 |
scrambled siRNA biological replicate 3 |
|
| Relations |
| BioProject |
PRJNA157829 |
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