|
| Status |
Public on Oct 25, 2012 |
| Title |
LIN28A-mediated regulation of microRNA biogenesis in mouse embryonic stem cell (A3-1) [Agilent] |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
LIN28A is a highly-conserved RNA-binding protein which is known to be involved in embryonic development, stem cell maintenance and proliferation. LIN28A is expressed in various types of cancer, and they are associated with advanced tumor malignancy. In embryonic stem cell, LIN28A specifically binds to let-7 precursors to suppress biogenesis of the let-7 microRNA family. In addition, LIN28A was reported to bind several mRNAs such as Oct4, cyclin A/B and histone H2A to activate their translation. For comprehensive understanding of the interaction between LIN28A and their target RNAs, we exploited UV-crosslinking and immunoprecipitation (CLIP) to capture their in vivo binding to target RNAs. LIN28A-binding RNAs were identified in a mouse embryonic stem cell line using multiple monoclonal and polyclonal antibodies. The result shows that LIN28 preferentially binds to let-7 precursors through GGAG binding motif, which is consistent with our previous results. We also identified that LIN28A binding is enriched in a certain subset of mRNAs. To understand the function of the novel LIN28A-mRNA binding, we carried out ribosome profiling from LIN28A-depleted mouse embryonic stem cells.
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| |
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| Overall design |
Examination of miRNA level in embryonic stem cell treated with siRNA for GFP or for Lin28a
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| |
|
| Contributor(s) |
Ha M, Chang H, Kim VN |
| Citation(s) |
23102813 |
| |
| Submission date |
Apr 09, 2012 |
| Last update date |
Jan 30, 2018 |
| Contact name |
Hyeshik Chang |
| E-mail(s) |
hyeshik@snu.ac.kr
|
| Organization name |
Seoul National University
|
| Department |
School of Biological Sciences
|
| Lab |
Hyeshik Chang Lab
|
| Street address |
Building 203 Room 525, School of Biological Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu
|
| City |
Seoul |
| State/province |
South Korea |
| ZIP/Postal code |
08826 |
| Country |
South Korea |
| |
|
| Platforms (1) |
| GPL8824 |
Agilent-019119 Mouse miRNA Microarray 1.0 G4472A (miRNA ID version) |
|
| Samples (4)
|
| GSM910958 |
A3-1 miRNA microarray - siGFP rep1 |
| GSM910959 |
A3-1 miRNA microarray - siGFP rep2 |
| GSM910960 |
A3-1 miRNA microarray - siLin28a rep1 |
| GSM910961 |
A3-1 miRNA microarray - siLin28a rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE37114 |
LIN28A targets, regulation of microRNA biogenesis, and effect on transcriptome in A3-1 cells |
|
| Relations |
| BioProject |
PRJNA158353 |
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