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| Status |
Public on May 09, 2012 |
| Title |
Human cerebrospinal fluid autoantibody lipid microarray profiling (Fig. 2A) |
| Platform organisms |
Pseudomonas aeruginosa; Escherichia coli; Staphylococcus aureus; Streptococcus pyogenes; Bacillus subtilis; Mycobacterium tuberculosis; Homo sapiens; Sus scrofa domesticus; Bos primigenius; Salmonella enterica subsp. enterica serovar Typhimurium |
| Sample organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
Lipids comprise 70% of the myelin sheath, and autoantibodies against lipids may contribute to the demyelination that characterizes multiple sclerosis (MS). We used lipid antigen microarrays and lipid mass spectrometry to identify bona fide lipid targets of the autoimmune response in MS brain and an animal model of MS to explore the role of the identified lipids in autoimmune demyelination. We found that autoantibodies in MS target a phosphate group in phosphatidylserine and oxidized phosphatidylcholine derivatives. Administration of these lipids ameliorated experimental autoimmune encephalomyelitis by suppressing activation and inducing apoptosis of autoreactive T cells, effects mediated by the lipids' saturated fatty-acid side chains. Thus, phospholipids represent a natural anti-inflammatory class of compounds that have potential as novel therapeutics for MS.
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| Overall design |
Fig. 2A. Mini-Array I: IgG antibody reactivity to various glycero-3-phosphocholine lipids in CSF samples from patients with relapsing remitting MS and from control patients with other neurological disease. Lipid hits with the lowest FDR (q=0.029) were clustered according to their reactivity profiles.
47 different lipids were custom-spotted in duplicate using the CAMAG Automatic TLC Sampler (ATS4) robot to spray 200 nl of 10 to 100 pmol of lipids onto PVDF membranes affixed to the surface of microscope slides. The slides were probed with cerebrospinal fluid (CSF) from 24 human patient samples. 25 slides total: 13 relapsing-remitting MS, 11 other neurological disease, and 1 secondary Ab alone (not included in this submission). CSF diluted 1/20. HRP-conjugated secondary Ab (goat anti-human IgM/IgG) diluted 1/175. ECL for 3 minutes.
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| Contributor(s) |
Ho PP, Kanter JL, Johnson AM, Narayana S, Khademi M, Olsson T, Steinman L, Robinson WH |
| Citation(s) |
22674551 |
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| Submission date |
Apr 30, 2012 |
| Last update date |
Jul 10, 2012 |
| Contact name |
William Robinson |
| E-mail(s) |
wrobins@stanford.edu
|
| Phone |
650-849-1207
|
| Fax |
650-849-1208
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| Organization name |
Stanford University
|
| Street address |
269 Campus Drive
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE37830 |
Human cerebrospinal fluid autoantibody lipid microarray profiling |
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| Relations |
| BioProject |
PRJNA164733 |
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