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Status |
Public on Dec 09, 2005 |
Title |
Global Gene Expression Profiles of Single-Cell Derived Fetal Rat Calvaria Cell Colonies with Adipocyte and Bone Markers |
Organism |
Rattus norvegicus |
Experiment type |
Expression profiling by array
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Summary |
Single cell-derived colonies were obtained by sorting 21 days fetal Rat calvaria cells into microtitre wells using flow cytometry. Colonies were categorized according to a limited gene expression profiling for a specific bone (osteocalcin) and a specific adipocyte (adipsin) marker by real-time PCR. Colonies expressing neither, one or both bone and adipocyte markers are in the sample set. Microarray analysis will address the global gene expression profiles of CFU-O (bone marker only), CFU-F (neither bone nor adipocyte marker) and CFU-bipotential (both markers) derived from single cells. CFU-A (adipocyte marker only) samples are also available for analysis if warranted. Keywords: other
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Overall design |
this experiment include 3 samples and 18 replicates
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Web link |
http://www.stembase.ca/?path=/browse/experiment&id=179
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Citation missing |
Has this study been published? Please login to update or notify GEO. |
Submission date |
Dec 07, 2005 |
Last update date |
Mar 03, 2017 |
Organization |
Ottawa Hospital Research Institute |
Phone |
(613) 737-8899 -73255
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Department |
Cellular and Molecular Medicine
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Lab |
Ottawa Bioinformatics Core Facility
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Street address |
501 Smyth Rd.
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City |
Ottawa |
State/province |
ON |
ZIP/Postal code |
K1H 8L6 |
Country |
Canada |
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Platforms (2) |
GPL341 |
[RAE230A] Affymetrix Rat Expression 230A Array |
GPL342 |
[RAE230B] Affymetrix Rat Expression 230B Array |
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Samples (18)
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GSM86604 |
JEACFU-O-II, chip RAE230B |
GSM86605 |
JEACFU-O-III, chip RAE230A |
GSM86606 |
JEACFU-O-III, chip RAE230B |
GSM86607 |
JEACFU-Bi-I, chip RAE230A |
GSM86608 |
JEACFU-Bi-I, chip RAE230B |
GSM86609 |
JEACFU-Bi-II, chip RAE230A |
GSM86610 |
JEACFU-Bi-II, chip RAE230B |
GSM86611 |
JEACFU-Bi-III, chip RAE230A |
GSM86612 |
JEACFU-Bi-III, chip RAE230B |
GSM86613 |
JEACFU-F-I, chip RAE230A |
GSM86614 |
JEACFU-F-I, chip RAE230B |
GSM86615 |
JEACFU-F-II, chip RAE230A |
GSM86616 |
JEACFU-F-II, chip RAE230B |
GSM86617 |
JEACFU-F-III, chip RAE230A |
GSM86618 |
JEACFU-F-III, chip RAE230B |
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Relations |
BioProject |
PRJNA93963 |