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| Status |
Public on Aug 05, 2015 |
| Title |
Transcriptional profile of Escherichia coli K12 strain DH10B harboring A1501 nitrogen fixation island (NFI) under nitrogen fixation conditions |
| Platform organism |
Escherichia coli |
| Sample organism |
Escherichia coli str. K-12 substr. DH10B |
| Experiment type |
Expression profiling by array
|
| Summary |
A1501 NFI is a genomic island derived from Pseudomonas stutzeri A1501. To study the molecular interactions of the P. stutzeri nif genes with the E. coli genome during nitrogen fixation, the NIF of A1501 was transferred into E. coli and comparative transcriptomics analyses were performed between nitrogen fixation conditions and nitrogen excess conditions.
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| Overall design |
RNA was extracted from E. coli K-12 DH10B cells with NFI cultured 20 h in NFDM medium under nitrogen fixation conditions (30℃, anaerobic, no ammonium) or nitrogen excess conditions (30℃, anaerobic, 20 mmol ammonium).
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| Contributor(s) |
Lin M, Han Y, Yan Y, Zhan Y, Li L |
| Citation(s) |
30026566 |
| |
| Submission date |
May 04, 2012 |
| Last update date |
Jul 26, 2019 |
| Contact name |
yongliang yan |
| E-mail(s) |
yongliangyan@yahoo.com.cn
|
| Organization name |
CAAS
|
| Street address |
zhongguancun south 12th
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| City |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
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| Platforms (1) |
| GPL3154 |
[E_coli_2] Affymetrix E. coli Genome 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA163265 |
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