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| Status |
Public on Mar 01, 2013 |
| Title |
microRNA binding by wildtype Ago2 and mutant Ago2 in human cells |
| Platform organisms |
Caenorhabditis elegans; Drosophila melanogaster; Danio rerio; Homo sapiens; Mus musculus; Rattus norvegicus |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Ago2 binds mature microRNAs (miRNAs) to regulate gene expression. A mutant Ago2 is constructed to render it unable to bind miRNAs and inhibit mRNA translation.
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| Overall design |
Human 293T cells were transiently transfected with a plasmid that encoded a FLAG-tagged wildtype human Ago2 or mutant Ago2. FLAG IP was preformed, and assoicated RNA was isolated and subjected miRNA microarray analysis.
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| Contributor(s) |
Zeng Y |
| Citation missing |
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| |
| Submission date |
May 11, 2012 |
| Last update date |
Mar 03, 2013 |
| Contact name |
Yan Zeng |
| E-mail(s) |
zengyan@njau.edu.cn
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| Organization name |
Nanjing Agricultural University
|
| Street address |
XuanWu District, No.1 WeiGang
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| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210095 |
| Country |
China |
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| Platforms (1) |
| GPL15559 |
University of Minnesota Custom miRNA microarray |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA165711 |
