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Series GSE37942 Query DataSets for GSE37942
Status Public on May 15, 2012
Title Comparison of gene expression during meiosis between wild-type and rad50-deficient strains of Coprinopsis cinerea
Organism Coprinopsis cinerea
Experiment type Expression profiling by array
Summary Meiosis was compared in rad50-1 and wild type strains of C. cinerea at 6 time points spanning the meiotic timecourse.
Abstract: The basidiomycete Coprinopsis cinerea is well-suited to studies of meiosis because meiosis progresses synchronously in ten million cells within each mushroom cap. Approximately 20% of C. cinerea genes exhibit changing expression during meiosis, but meiosis and mushroom development happen concurrently so differentially expressed genes might not be directly involved in meiotic processes. Using microarrays, we examined global gene expression across a meiotic time course in two mutants in which meiosis arrests but mushrooms develop normally. Genes differentially expressed in the mutants compared to wild type are likely to be involved in meiosis and sporulation as opposed to mushroom development. In rad50-1, which arrests in late prophase, RNA abundance for a group of early meiotic genes remains high, while the expression of a group of late meiotic genes is never induced. In contrast, in msh5-22 (which fails to undergo pre-meiotic DNA replication), both early and late meiotic genes are underexpressed relative to wild type at late meiotic time points as the cells die. Genes that are differentially expressed in both mutants are particularly strong candidates for playing roles in meiosis and sporulation.
 
Overall design Five time points were analyzed, with four biological replicate rad50-1 samples used for each timepoint. Reference wild-type samples consisted of pooled RNA from ten samples at the appropriate timepoint.
 
Contributor(s) Anderson E, Burns C, Zolan ME
Citation(s) 23050232
Submission date May 11, 2012
Last update date Nov 06, 2012
Contact name Claire Burns
E-mail(s) burnsc@indiana.edu
Organization name Indiana University
Department Biology
Lab Zolan Lab
Street address 1001 E 3rd St
City Bloomington
State/province IN
ZIP/Postal code 47404
Country USA
 
Platforms (1)
GPL7697 IU/UNC Coprinopsis cinerea
Samples (20)
GSM930427 Gill_tissue_K_Rep1_rad50
GSM930428 Gill_tissue_K_Rep2_rad50
GSM930429 Gill_tissue_K_Rep3_rad50
This SubSeries is part of SuperSeries:
GSE37968 omparison of gene expression during meiosis between wild-type and deficient strains of Coprinopsis cinerea
Relations
BioProject PRJNA167156

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37942_RAW.tar 28.7 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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