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Status |
Public on Dec 31, 2014 |
Title |
CD44-high Cancer stem-cell like cell fraction in transformed human esophageal cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Comparison of CD44-high/CD24-low (CD44H) and CD44-low/CD24-high (CD44L) cell fractions in transformed human esophageal cell line EPC2-T CD44H cells display enhanced malignant potential (e.g. tumorigenicity, colony formation) compared to CD44L cells.
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Overall design |
Transformed human esophageal keratinocyte cell line EPC2-T (EPC2-hTERT-EGFR-cyclin D1-p53R175H) appeared to contain two cell populations defined by high expression of CD44 (CD44H) as well as low expression of CD44 (CD44L). The former (CD44H) displayed fibroblast-like mesenchymal morphology while the latter (CD44L) remained cuboidal epithelioid morphology in culture. CD44H and CD44L cells were sorted by fluorescence-activated cell sortingand subjected to RNA extraction and gene array experiments.
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Contributor(s) |
Nakagawa H, Rustgi AK |
Citation missing |
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Submission date |
May 15, 2012 |
Last update date |
Jul 26, 2018 |
Contact name |
Hiroshi Nakagawa |
E-mail(s) |
nakagawh@mail.med.upenn.edu
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Phone |
215-573-1867
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Organization name |
University of Pennsylvania
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Department |
Gastroenterology Division
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Lab |
Nakagawa
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Street address |
415 Curie Blvd.
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City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19104 |
Country |
USA |
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Platforms (1) |
GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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Samples (6)
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GSM931650 |
CD24-high/CD44-low (CD44L) EPC2 cell fraction 44L1 |
GSM931651 |
CD24-high/CD44-low (CD44L) EPC2 cell fraction 44L2 |
GSM931652 |
CD24-high/CD44-low (CD44L) EPC2 cell fraction 44L3 |
GSM931653 |
CD24-low/CD44-high (CD44H) EPC2 cell fraction 44H1 |
GSM931654 |
CD24-low/CD44-high (CD44H) EPC2 cell fraction 44H2 |
GSM931655 |
CD24-low/CD44-high (CD44H) EPC2 cell fraction 44H3 |
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Relations |
BioProject |
PRJNA167081 |