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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 01, 2006 |
| Title |
Comparison between naïve, tolerant and memory CD8+ T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To elucidate transcriptional differences between naïve, tolerant and memory CD8+ T cells, genome-wide gene expression profiling was performed. The indicated cell populations were purified from immunized or naïve Rag-/- TCR mice by cell sorting and RNA was extracted labeled and hybridized to an Affymetrix custom mouse array GNF1M GeneChip. RNA from sorted samples was prepared, split into two aliquots prior to making labeled cDNA, so that two arrays were used for each cell population.Alterations in transcript levels were determined through pair wise comparisons of naïve cells to either memory or tolerant CD8+ T cells.
Keywords: cell type
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| Overall design |
duplicates consisted of replicate aliquots of the same total RNA
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| Contributor(s) |
Teague RM, Sather BD, Sacks J, Huang MZ, Dossett ML, Morimoto J, Sutton S, Cooke MP, Tan X, Öhlén C, Greenberg PD |
| Citation(s) |
16474399 |
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| Submission date |
Dec 09, 2005 |
| Last update date |
Mar 16, 2012 |
| Contact name |
John R Walker |
| E-mail(s) |
jwalker@gnf.org
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| Phone |
858-812-1636
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| Organization name |
Genomics Institute of the Novartis Research Foundation
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| Lab |
Genetics Core
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| Street address |
10675 John Jay Hopkins
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| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
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| Platforms (1) |
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| Samples (6)
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| GSM87300 |
naive TCR1;CD8+T cells;NONE;C57/BL6,gag-specific TCR;29779 |
| GSM87301 |
naive TCR2;CD8+T cells;NONE;C57/BL6,gag-specific TCR;29780 |
| GSM87302 |
memory TCR1;CD8+, memory;NONE;C57/BL6,gag-specific TCR;29781 |
| GSM87303 |
memory TCR2;CD8+, memory;NONE;C57/BL6,gag-specific TCR;29782 |
| GSM87304 |
tolerant TCR1;CD8+, tolerant;NONE;C57/BL6,gag-specific ;29783 |
| GSM87305 |
tolerant TCR2;CD8+, tolerant;NONE;C57/BL6,gag-specific ;29784 |
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| Relations |
| BioProject |
PRJNA94003 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE3800_RAW.tar |
56.4 Mb |
(http)(custom) |
TAR (of CEL) |
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