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| Status |
Public on Jun 17, 2013 |
| Title |
Transcriptomic profiles of zebrafish embryos exposed to silver (nanoparticles, bulk and ions) using HT-SuperSAGE in a Illumina GA2 platform |
| Organism |
Danio rerio |
| Experiment type |
Other
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| Summary |
Silver nanoparticles cause toxicity in exposed organisms and are an environmental health concern. The mechanisms of silver nanoparticle toxicity, however, remain unclear. We examined the effects of exposure to silver in nano-, bulk- and ionic forms on zebrafish embryos (Danio rerio) using a Next Generation Sequencing approach in an Illumina platform (High-Throughput SuperSAGE). Significant alterations in gene expression were found for all treatments and many of the gene pathways affected, most notably those associated with oxidative phosphorylation and protein synthesis, overlapped strongly between the three treatments indicating similar mechanisms of toxicity for the three forms of silver studied. Changes in oxidative phosphorylation indicated a down-regulation of this pathway at 24h of exposure, but with a recovery at 48h. This finding was consistent with a dose-dependent decrease in oxygen consumption at 24h, but not at 48h, following exposure to silver ions. Overall, our data provide support for the hypothesis that the toxicity caused by silver nanoparticles is principally associated with bioavailable silver ions in exposed zebrafish embryos. These findings are important in the evaluation of the risk that silver particles may pose to exposed vertebrate organisms.
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| Overall design |
mRNA profiles of whole zebrafish embryos at 24 and 48 hours post-fertilisation (hpf) exposed to silver in nano, bulk and ionic forms were generated by deep sequencing using HT-SuperSAGE (Illumina GA2).
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| Contributor(s) |
van Aerle R, Lange A, Moorhouse A, Paszkiewicz K, Ball K, Johnston BD, de-Bastos E, Booth T, Tyler CR, Santos EM |
| Citation(s) |
23758687 |
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| Submission date |
May 22, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Ronny van Aerle |
| E-mail(s) |
r.van-aerle@exeter.ac.uk
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| Organization name |
University of Exeter
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| Department |
Biosciences, College of Life and Environmental Sciences
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| Lab |
van Aerle Lab
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| Street address |
Stocker Road
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| City |
Exeter |
| State/province |
Devon |
| ZIP/Postal code |
EX4 4QD |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL9319 |
Illumina Genome Analyzer II (Danio rerio) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA167302 |
| SRA |
SRP013309 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38125_RAW.tar |
8.5 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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