|
| Status |
Public on Aug 27, 2012 |
| Title |
Guthrie card methylomics identifies temporally stable epialleles that are present at birth in humans (Bisulfite-Seq) |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
A major concern in common disease epigenomics is distinguishing causal from consequential epigenetic variation. One means of addressing this issue is to identify the temporal origins of epigenetic variants via longitudinal analyses. However, prospective birth-cohort studies are expensive and time-consuming. Here we report DNA methylomics of archived Guthrie cards for the retrospective longitudinal analyses of in utero-derived DNA methylation variation. We first validate two methodologies for generating comprehensive DNA methylomes from Guthrie cards. Then, using an integrated epigenomic/genomic analysis of Guthrie cards and follow-up samplings, we identify inter-individual DNA methylation variation that is present both at birth and three years later. These findings suggest that disease-relevant epigenetic variation could be detected at birth i.e. before overt clinical disease. Guthrie card methylomics offers a potentially powerful and cost-effective strategy for studying the dynamics of inter-individual epigenomic variation in a range of common human diseases.
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| |
|
| Overall design |
Bisulphite converted DNA was sequenced
|
| |
|
| Contributor(s) |
Beyan H, Down TA, Ramagopalan SV, Uvebrant K, Nilsson A, Holland ML, Gemma C, Giovannoni G, Boehm BO, Ebers GC, Lernmark A, Cilio CM, Leslie RD, Rakyan VK |
| Citation(s) |
22919074 |
| |
| Submission date |
May 22, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Thomas A Down |
| E-mail(s) |
thomas.down@gurdon.cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
WT/CRUK Gurdon Institute
|
| Street address |
Tennis Court Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 1QN |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (6)
|
| GSM935138 |
genomic DNA from sample F1 dried blood spot (Bisulfite-Seq) |
| GSM935139 |
genomic DNA from sample F2 dried blood spot (Bisulfite-Seq) |
| GSM935140 |
genomic DNA from sample M dried blood spot (Bisulfite-Seq) |
| GSM935141 |
genomic DNA from sample F1 follow-up (Bisulfite-Seq) |
| GSM935142 |
genomic DNA from sample F2 follow-up (Bisulfite-Seq) |
| GSM935143 |
genomic DNA from sample M follow-up (Bisulfite-Seq) |
|
| This SubSeries is part of SuperSeries: |
| GSE38128 |
Guthrie card methylomics identifies temporally stable epialleles that are present at birth in humans |
|
| Relations |
| BioProject |
PRJNA167305 |
| SRA |
SRP013310 |
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