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Series GSE3816 Query DataSets for GSE3816
Status Public on Jan 25, 2006
Title Sporulation_transfer_to_YPD
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary We have used genome-wide expression profiling to investigate how budding yeast cells become committed to sporulation. Sporulating cells were transferred to growth medium at different stages of the process, and their transcription response was characterized. Most sporulation-induced genes were immediately down-regulated upon the transfer, even in committed cells that continued to sporulate. The metabolic-related transcription response of pre-committed cells or of mature spores transferred to growth medium was essentially the same as that of vegetative cells exposed to glucose. In contrast, committed cells elicited a unique, and dramatically different response. Our results suggest that cells ensure commitment to sporulation not by stabilizing the process, but by modulating their information processing in an active manner that may optimize sporulation in an environment-specific manner.
Keywords: Time course
 
Overall design Wild type SK1 cells (NKY1551) grown to saturation in YPD (2% yeast extract, 4% bactopeptone, 4% glucose) for 24 hours, diluted into YPA (1% yeast extract, 2% bactopeptone 1% Potassium Acetate) and grown overnight. Part of that culture was used as reference for all hybridizations preformed in this study. The cells were then washed twice with sterile water and resuspended in SPM media (0.3% Potassium Acetate and 0.02% Rafinose) to initiate the sporulation process. At different stages of the sporulation process (2, 3, 4, 5, 6, 7, 8, 9 and 10 hours) RNA was extracted for microarray hybridizations. At each hour, part of the culture was centrifuged and resuspended in YPD. Each YPD culture was monitored immediately after the transfer, after 20 and 40 minutes.
 
Citation(s) 16542486
Submission date Dec 13, 2005
Last update date Mar 16, 2012
Contact name Naama Barkai
E-mail(s) naama.barkai@weizmann.ac.il
Organization name Weizmann Institute
Street address P.O box 26
City Rehovot
ZIP/Postal code 76100
Country Israel
 
Platforms (2)
GPL3242 UHN Yeast 6.4k1v2
GPL3243 UHN Yeast 6.4kv2
Samples (36)
GSM87588 Sporulation_2h (6.4k1v2 array)
GSM87589 Sporulation_3h (6.4k1v2 array)
GSM87590 Sporulation_4h (6.4kv2 array)
Relations
BioProject PRJNA94027

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE3816_RAW.tar 85.8 Mb (http)(custom) TAR (of TXT)

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