|
Status |
Public on Jan 15, 2013 |
Title |
Genome-wide identification of Ikaros binding sites in pre-B cells |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
Summary |
Ikaros DNA binding proteins are important regulators of haematopoiesis and genetic deletion of Ikaros results in severe developmental disturbances, including delayed thymocyte differentiation and an early and complete block in B cell development. Although Ikaros ChIP-seq data are available for mouse thymocytes and human haematopoietic progenitors, it has not been achieved in B cell progenitors. The goal of this study was to identify Ikaros binding sites in pre-B cells to define Ikaros target genes which could explain the essential role of Ikaros proteins in B cell differentiation.
|
|
|
Overall design |
We carried out chromatin immunoprecipitation and high throughput sequencing (ChIP-seq) with antibodies to the C-terminus of endogenous Ikaros in B3 cells and with anti-haemagglutinin (HA) in B3 cells transduced with epitope-tagged HA-Ikaros.
|
|
|
Contributor(s) |
Ferreirós Vidal I, Terry A, Merkenschlager M |
Citation(s) |
23303821 |
|
Submission date |
May 23, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Gopuraja Dharmalingam |
Organization name |
MRC London Institute of Medical Sciences
|
Department |
Epigenetics section
|
Street address |
Faculty of Medicine, Imperial College, Hammersmith Hospital Campus
|
City |
London |
ZIP/Postal code |
W12 0NN |
Country |
United Kingdom |
|
|
Platforms (1) |
GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
|
Samples (7)
|
|
This SubSeries is part of SuperSeries: |
GSE38200 |
Ikaros target genes in the mouse pre-B cell line B3 |
|
Relations |
SRA |
SRP013344 |
BioProject |
PRJNA167357 |