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Status |
Public on Aug 01, 2012 |
Title |
Gene expression profiling and pathway analysis of human bronchial epithelial cells exposed to airborne particulate matter collected from Saudi Arabia |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Whole transcript expression was profiled using the Affymetrix 1.0 array in human bronchial epithelial cells exposed to PM collected from Saudi Arabia for 1 or 4 days. The differentially expressed genes were identified and analyzed for enriched networks and pathways using Ingenuity Pathway Analysis (IPA). We have identified 140 and 230 genes that significantly changed more than 1.5 fold after PM exposure for 1 or 4 days, respectively. IPA analysis revealed that different exposure durations triggered distinct pathways. Genes involved in NRF2-mediated response to oxidative stress were up-regulated after 1 day exposure. In contrast, cells exposed for 4 days exhibited significantly changes in genes related to cholesterol and lipid synthesis pathways.
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Overall design |
We analyzed gene expression profiles from 12 samples collected at two different time points, including 2 untreated controls, 2 normal PM treated samples and 2 storm PM treated samples for each time point.
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Contributor(s) |
Sun H, Shamy M, Kluz T, Zhong M, Laulicht F, Alghamdi MA, Khoder MI, Chen L, Costa M |
Citation missing |
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Submission date |
May 23, 2012 |
Last update date |
Jul 26, 2018 |
Contact name |
Hong Sun |
Organization name |
NYU School of Medicine
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Department |
Medicine
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Lab |
Sun Lab
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Street address |
341 East 25 Street
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10010 |
Country |
USA |
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Platforms (1) |
GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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Samples (12)
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GSM936215 |
untreated control samples collected at D1 #1 |
GSM936216 |
untreated control samples collected at D1 #2 |
GSM936217 |
normal PM treated samples collected at D1 after exposure #1 |
GSM936218 |
normal PM treated samples collected at D1 after exposure #2 |
GSM936219 |
storm PM treated samples collected at D1 after exposure #1 |
GSM936220 |
storm PM treated samples collected at D1 after exposure #2 |
GSM936221 |
untreated control samples collected at D4 #1 |
GSM936222 |
untreated control samples collected at D4 #2 |
GSM936223 |
normal PM treated samples collected at D4 after exposure #1 |
GSM936224 |
normal PM treated samples collected at D4 after exposure #2 |
GSM936225 |
storm PM treated samples collected at D4 after exposure #1 |
GSM936226 |
storm PM treated samples collected at D4 after exposure #2 |
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Relations |
BioProject |
PRJNA167360 |