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Series GSE38175 Query DataSets for GSE38175
Status Public on Aug 01, 2012
Title The histone demethylase PHF8 is essential for cytoskeleton dynamics
Organism Homo sapiens
Experiment type Expression profiling by array
Summary PHF8 is a histone demethylase associated with X-linked mental retardation (XLMR). It has been described as a transcriptional coactivator involved in cell cycle progression, but its physiological role is still poorly understood. Here we show that PHF8 controls the expression of genes involved in cell adhesion and cytoskeleton organization such as RhoA, Rac1 and GSK3β. A lack of PHF8 not only results in a cell cycle delay but also in a disorganized actin cytoskeleton and impaired cell adhesion. Our data demonstrate that PHF8 directly regulates the expression of these genes by demethylating H4K20me1 at promoters. Moreover, c-Myc transcription factor interacts with PHF8 and binds to the analyzed promoters, suggesting that c-Myc is involved on PHF8 recruitment to these promoters. Further analysis in the neuroblastoma cell line SH-SY5Y and in cortical neurons shows that depletion of PHF8 results in deficient neurite elongation. Overall, our results suggest that the mental retardation phenotype associated with loss of function of PHF8 could be due to abnormal neuronal connections as a result of alterations in cytoskeleton function.
 
Overall design HeLa cells were transfected with either control or PHF8 siRNAs. Experiments were performed in biological triplicates. RNA were extracted and sujected to microarray analysis.
 
Contributor(s) Asensio-Juan E, Gallego C, Martínez-Balbás M
Citation(s) 22850744
Submission date May 23, 2012
Last update date Feb 18, 2019
Contact name Elena Asensio
Organization name IBMB_CSIC
Department Molecular Genomics
Street address Baldiri Reixac 4-8
City Barcelona
State/province Select a State or Province
ZIP/Postal code 08028
Country Spain
 
Platforms (1)
GPL6884 Illumina HumanWG-6 v3.0 expression beadchip
Samples (6)
GSM936227 Control shRNA1
GSM936353 Control shRNA2
GSM936354 Control shRNA3
Relations
BioProject PRJNA167332

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38175_RAW.tar 6.3 Mb (http)(custom) TAR
GSE38175_non-normalized.txt.gz 13.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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