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Series GSE3818 Query DataSets for GSE3818
Status Public on Jan 25, 2006
Title Sporulation_SPS4_NDT80_transfer_to_YPD
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary We have used genome-wide expression profiling to investigate how budding yeast cells become committed to sporulation. Sporulating cells were transferred to growth medium at different stages of the process, and their transcription response was characterized. Most sporulation-induced genes were immediately down-regulated upon the transfer, even in committed cells that continued to sporulate. The metabolic-related transcription response of pre-committed cells or of mature spores transferred to growth medium was essentially the same as that of vegetative cells exposed to glucose. In contrast, committed cells elicited a unique, and dramatically different response. Our results suggest that cells ensure commitment to sporulation not by stabilizing the process, but by modulating their information processing in an active manner that may optimize sporulation in an environment-specific manner.
Keywords: Time course
 
Overall design SK1 cells, in which NDT80 is expressed under the promoter of SPS4 (GF18), were grown to saturation in YPD (2% yeast extract, 4% bactopeptone, 4% glucose) for 24 hours, diluted into YPA (1% yeast extract, 2% bactopeptone 1% Potassium Acetate) and grown overnight. The cells were then washed twice with sterile water and resuspended in SPM media (0.3% Potassium Acetate and 0.02% Rafinose) to initiate the sporulation process. At 5.5 hours of the sporulation process, cells were kept for RNA extraction. Part of the culture was centrifuged and resuspended in YPD, and was monitored immediately, 20 and 40 minutes after the transfer.
 
Citation(s) 16542486
Submission date Dec 13, 2005
Last update date Mar 16, 2012
Contact name Naama Barkai
E-mail(s) naama.barkai@weizmann.ac.il
Organization name Weizmann Institute
Street address P.O box 26
City Rehovot
ZIP/Postal code 76100
Country Israel
 
Platforms (1)
GPL3245 UHN Yeast 6.4kv4
Samples (4)
GSM87627 Sporulation_5.5h (6.4kv4 array)
GSM87628 RTG_5.5R0 (6.4kv4 array)
GSM87629 RTG_5.5R20 (6.4kv4 array)
Relations
BioProject PRJNA94031

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE3818_RAW.tar 8.1 Mb (http)(custom) TAR (of TXT)

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