|
| Status |
Public on May 24, 2012 |
| Title |
Synthetic circuits for tracking human cell fate |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Cells respond heterogeneously to DNA damage. We engineered genetic circuits to detect differential responses in a population that persist for many days post-stimulus.
We used microarrays to compare memory and non-memory subpopulations 3 days after DNA damage or doxycycline exposure.
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| |
|
| Overall design |
MD12/p53R2-RE and MD10/TetOx2 cells were either exposed to UV (10uJ/m^2) or doxycycline (1 ug/mL, 24 hours) and allowed to recover 3 days before sortng of memory and non-memory cells and RNA extraction. Two replicates were submitted for each condition (UV memory, UV non-memory, dox memory, dox non-memory)
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| |
|
| Contributor(s) |
Burrill DR, Inniss MC, Boyle PM, Silver PA |
| Citation(s) |
22751502 |
| Submission date |
May 23, 2012 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Mara Christine Inniss |
| E-mail(s) |
minniss@fas.harvard.edu
|
| Organization name |
Harvard Medical School
|
| Department |
Systems Biology
|
| Lab |
Silver lab
|
| Street address |
200 Longwood Ave.
|
| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA167441 |
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