NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE38257 Query DataSets for GSE38257
Status Public on Dec 21, 2012
Title A Novel Tumor suppressor network in squamous malignancies
Organism Mus musculus
Experiment type Expression profiling by array
Summary The specific ablation of Rb1 gene in stratified epithelia (RbF/F;K14cre) promotes proliferation and altered differentiation but is insufficient to produce spontaneous tumors. The pRb relative, p107, compensates some of the functions of pRb in these tissues, however RbF/F;K14cre;p107-/- mice die postnatally. Acute pRb loss in stratified epithelia, using an inducible mouse model (RbF/F;K14creERTM), shows that p107 exerts specific tumor suppressor functions in its absence. After simultaneous absence of pRb and p107, p53 transcriptional function is impaired and Pten expression is reduced. All mutant mice develop spontaneous squamous tumors carcinomas rapidly. Gene expression analysis of mouse tumors, besides supporting the impaired p53 function and the susceptibility to Akt/mTOR inhibitors, also revealed significant overlap with human squamous carcinomas. Thus, RbF/F;K14creERTM;p107-/- may constitute a new mouse model for these malignancies. Collectively, these data demonstrate the existence of a previously unreported functional connection between pRb, Pten and p53 tumor suppressors, through p107, of a particular relevance in squamous tumor development.
 
Overall design Gene expression was compared between normal mouse skin and carcinomas arising in the skin of RbF/F;K14creERTM;p107-/- mouse. All mice were treated with tamoxifen.
 
Contributor(s) Costa C, Santos M, Segrelles C, Duenas M, Lara MF, Xabier A, Garcia-Escudero R, Paramio JM
Citation(s) 23145321
Submission date May 25, 2012
Last update date Feb 11, 2019
Contact name Ramon Garcia-Escudero
E-mail(s) ramon.garcia@ciemat.es, rgescudero@gmail.com
Organization name CIEMAT
Department Biomedical Innovation
Lab Molecular and Traslational Oncology
Street address Avda Complutense 40, Ed 70A
City Madrid
State/province Madrid
ZIP/Postal code 28040
Country Spain
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (14)
GSM937546 Skin_Normal_1
GSM937547 Skin_Normal_2
GSM937548 Skin_Normal_3
Relations
BioProject PRJNA167525

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38257_RAW.tar 49.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap