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Status |
Public on Dec 31, 2012 |
Title |
Genome-wide cistromes of thyroid hormone receptors alpha and beta in C17.2 cells |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Tagged versions of thyroid hormone receptors alpha (TRa) and beta (TRb) were stably transfected in two C17.2 cell lines, C17.2a and C17.2b, respectively. We performed an affinity-based purification of chromatin (ChAP), and high-throughput sequencing was used to assess binding sites of both receptors (ChAP-Seq). Standard ChIP-Seq for RXR was also performed in C17.2a cells. These data allow us to compare binding sites for both receptors and to conclude that they were only partially redundant, with co-existence of receptor-specific sites.
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Overall design |
Examination of binding sites of the two thyroid hormone receptors (alpha, beta) in two cell lines (C17.2a, C17.2b), each expressing one of the receptors. Examination of RXR binding sites in C17.2a cells.
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Contributor(s) |
Chatonnet F, Guyot R, Benoît G, Flamant F |
Citation(s) |
- Chatonnet F, Guyot R, Benoît G, Flamant F. Genome-wide analysis of thyroid hormone receptors shared and specific functions in neural cells. Proc Natl Acad Sci U S A 2013 Feb 19;110(8):E766-75. PMID: 23382204
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Submission date |
May 30, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Fabrice Chatonnet |
E-mail(s) |
fabrice.chatonnet@ens-lyon.org
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Phone |
+33 2 99 28 99 39
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Organization name |
University Hospital of Rennes
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Lab |
Inserm U1236 MICMAC
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Street address |
2 rue Henri le Guilloux
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City |
Rennes |
ZIP/Postal code |
35033 |
Country |
France |
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Platforms (1) |
GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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Samples (3) |
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This SubSeries is part of SuperSeries: |
GSE41727 |
Genome-wide analysis of thyroid hormone receptors' shared and specific functions in neural cells |
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Relations |
BioProject |
PRJNA167689 |
SRA |
SRP013451 |