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Series GSE38597

Query DataSets for GSE38597

Status

Public on Jun 09, 2012

Title

Gene expression profiling of 6 acute hepatitis C patients

Organism

Homo sapiens

Experiment type

Expression profiling by array
Third-party reanalysis

Summary

Approximately 50% of patients with chronic hepatitis C (CHC) have a sustained virologic response (SVR) to treatment with pegylated interferon (pegINF)-α and ribavirin. Non-response to treatment is associated with constitutively increased expression of IFN-stimulated genes (ISGs) in the liver. Treatment of patients with acute hepatitis C (AHC) is more effective, with SVR rates >90%. We investigated mechanisms of the different responses of patients with CHC and AHC to pegIFN-α therapy. We analyzed IFN signaling and ISG expression in liver samples from patients with acute hepatitis C (AHC), patients with chronic hepatitis (CHC), and individuals without hepatitis C (controls) using microarray, immunohistochemical, and protein analyses. Findings were compared with those from primary human hepatocytes stimulated with IFN-α or IFN-γ, as reference sets. Expression levels of 100s of genes, primarily those regulated by IFN-γ, were altered in liver samples from patients with AHC compared with controls. Expression of IFN-γ–stimulated genes was induced in liver samples from patients with AHC, whereas expression of IFN-α–stimulated genes was induced in samples from patients with CHC. In an expression analysis of negative regulators of IFN-α signaling, we did not observe differences in expression of SOCS1 or SOCS3 between liver samples from patients with AHC and those with CHC. However, USP18 (another negative regulator of IFN-α signaling), was upregulated in liver samples of patients with CHC that did not respond to therapy, but not in AHC. In conclusion, differences in expression of ISGs might account for the greater response of patients with AHC, compared to those with CHC, to treatment with pegINF-α and ribavirin. Specifically, USP18 is upregulated in liver samples of patients with CHC that do not respond to therapy, but not in patients with AHC.

(Interferon-γ Stimulated Genes, but not USP18, are Expressed in Livers of Patients with Acute Hepatitis C; Dill MT, Makowska Z et al, Gastroenterology 2012 (in press))

Overall design

Liver biopsy samples from 6 patients with acute hepatitis C virus infection were analyzed
CHC data and controls previously submitted as GSE11190

Contributor(s)

Dill MT, Makowska Z, Duong FT, Merkofer F, Filipowicz M, Baumert TF, Tornillo L, Terraciano L, Heim MH

Citation(s)

22677194

Submission date

Jun 08, 2012

Last update date

Mar 25, 2019

Contact name

Zuzanna Makowska

Organization name

University of Basel

Department

Biomedicine

Lab

Hepatology

Street address

Hebelstrasse 20

City

Basel

ZIP/Postal code

4031

Country

Switzerland

Platforms (1)

GPL570

[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

Samples (8)

More...

GSM945910	Acute hepatitis C patient 1
GSM945911	Acute hepatitis C patient 2
GSM945912	Acute hepatitis C patient 3

This SubSeries is part of SuperSeries:

GSE38598

Hepatocytes treated with IFN-alpha or IFN-gamma and acute hepatitis C

Relations

Reanalysis of

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BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE38597_GSE11190_RMA.txt.gz	7.6 Mb	(ftp)(http)	TXT
GSE38597_RAW.tar	33.2 Mb	(http)(custom)	TAR (of CEL)
GSE38597_readme.txt	1.5 Kb	(ftp)(http)	TXT
Processed data included within Sample table
Processed data are available on Series record