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| Status |
Public on Aug 23, 2012 |
| Title |
Gene expression profiling of CD4+myelin basic protein specific T cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
CNS autoimmunity is induced by autoreactive T cells reactive against CNS antigen. However how these T cells become able to transgress the blood brain barrier is not CNS autoimmunity is induced by autoreactive T cells reactive against CNS antigen. Here a gene expression profile of the pathogenic T cells in different functional states was performed.
These studies were performed in a classical model of multiple sclerosis, experimental autoimmune encephalomyelitis in Lewis rats induced by transfer of CD4+myelin basic protein specific T cells.
We found that on their way to the CNS T cells fundamentally reprogram their gene expression profile, by down-regulating their activation program and up-regulating cell locomotion molecules.
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| Overall design |
Total RNA extracted from ex vitro myelin specific T cells (blasts and resting state, day 2 and 7 after antigen challenge respectively) or isolated from the spleen (3 days p.t.) was used to perform a genome-wide transcriptional profiling assay (Rat Genome 230, Affymetrix)-
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| Contributor(s) |
Odoardi F, Ulaganathan VK, Lottaz C, Nietfeld W, Flügel A |
| Citation(s) |
22914092 |
| Submission date |
Jun 11, 2012 |
| Last update date |
Mar 03, 2017 |
| Contact name |
alexander flügel |
| E-mail(s) |
fluegel@med.uni-goettingen.de
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| Phone |
++49 (0)551 39 13332
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| Fax |
++49 (0)551 39 13348
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| Organization name |
Institute for Multiple Sclerosis Research
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| Department |
Department of Neuroimmunology
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| Street address |
waldweg 33
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| City |
Göttingen |
| ZIP/Postal code |
37073 |
| Country |
Germany |
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| Platforms (2) |
| GPL341 |
[RAE230A] Affymetrix Rat Expression 230A Array |
| GPL342 |
[RAE230B] Affymetrix Rat Expression 230B Array |
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| Samples (22)
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| GSM946766 |
Activated T cell blasts (2 days post invitro activation), replicate 1, Rat230_array-A |
| GSM946767 |
Activated T cell blasts (2 days post invitro activation), replicate 2, Rat230_array-A |
| GSM946768 |
Activated T cell blasts (2 days post invitro activation), replicate 3, Rat230_array-A |
| GSM946769 |
Activated T cell blasts (2 days post invitro activation), replicate 4, Rat230_array-A |
| GSM946770 |
Activated T cell blasts (2 days post invitro activation), replicate 5, Rat230_array-A |
| GSM946771 |
Activated T cell blasts (2 days post invitro activation), replicate 1, Rat230_array-B |
| GSM946772 |
Activated T cell blasts (2 days post invitro activation), replicate 2, Rat230_array-B |
| GSM946773 |
Activated T cell blasts (2 days post invitro activation), replicate 3, Rat230_array-B |
| GSM946774 |
Activated T cell blasts (2 days post invitro activation), replicate 4, Rat230_array-B |
| GSM946775 |
Activated T cell blasts (2 days post invitro activation), replicate 5, Rat230_array-B |
| GSM946776 |
Resting T cell (7 days post invitro activation), replicate 1, Rat230_array-A |
| GSM946777 |
Resting T cell (7 days post invitro activation), replicate 2, Rat230_array-A |
| GSM946778 |
Resting T cell (7 days post invitro activation), replicate 3, Rat230_array-A |
| GSM946779 |
Resting T cell (7 days post invitro activation), replicate 4, Rat230_array-A |
| GSM946780 |
Resting T cell (7 days post invitro activation), replicate 1, Rat230_array-B |
| GSM946781 |
Resting T cell (7 days post invitro activation), replicate 2, Rat230_array-B |
| GSM946782 |
Resting T cell (7 days post invitro activation), replicate 3, Rat230_array-B |
| GSM946783 |
Resting T cell (7 days post invitro activation), replicate 4, Rat230_array-B |
| GSM946784 |
Ex vivo sorted T cell from spleen (3 days post adoptive transfer), replicate 1, Rat230_array-A |
| GSM946785 |
Ex vivo sorted T cell from spleen (3 days post adoptive transfer), replicate 2, Rat230_array-A |
| GSM946786 |
Ex vivo sorted T cell from spleen (3 days post adoptive transfer), replicate 1, Rat230_array-B |
| GSM946787 |
Ex vivo sorted T cell from spleen (3 days post adoptive transfer), replicate 2, Rat230_array-B |
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| Relations |
| BioProject |
PRJNA168304 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38645_RAW.tar |
54.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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