|
| Status |
Public on Aug 27, 2012 |
| Title |
Id2a knockdown in zebrafish retina |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
During vertebrate retinogenesis, the precise balance between retinoblast proliferation and differentiation is spatially and temporally regulated through a number of intrinsic factors and extrinsic signaling pathways. Moreover, there are complex gene regulatory network interactions between these intrinsic factors and extrinsic pathways, which ultimately function to determine when retinoblasts exit the cell cycle and terminally differentiate. We recently uncovered a cell non-autonomous role for the intrinsic HLH factor, Id2a, in regulating retinoblast proliferation and differentiation, with Id2a-deficient retinae containing an abundance of proliferative retinoblasts and an absence of terminally differentiated retinal neurons and glia. Here, we report that Id2a function is necessary and sufficient to limit Notch pathway activity during retinogenesis. Id2a-deficient retinae possess elevated levels of Notch pathway component gene expression, while retinae overexpressing id2a possess reduced expression of Notch pathway component genes. Attenuation of Notch signaling activity by DAPT or by morpholino knockdown of Notch1a is sufficient to rescue both the proliferative and differentiation defects in Id2a-deficient retinae. In addition to regulating Notch pathway activity, through an RNA-Seq and differential gene expression analysis of Id2a-deficient retinae, we identify a number of additional intrinsic and extrinsic regulatory pathway components whose expression is regulated by Id2a. These data highlight the integral role played by Id2a in the gene regulatory network governing the transition from retinoblast proliferation to terminal differentiation during vertebrate retinogenesis.
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| |
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| Overall design |
Two biological replicates for both Id2aMM and Id2aMO samples
|
| |
|
| Contributor(s) |
Gross J, Uribe R, Marcotte EM, Kwon T |
| Citation(s) |
22981606 |
| |
| Submission date |
Jun 18, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Taejoon Kwon |
| E-mail(s) |
tkwon@unist.ac.kr
|
| Organization name |
Ulsan National Institute of Science and Technology
|
| Department |
Department of Biomedical Engineering
|
| Street address |
50 Unist-gil
|
| City |
Ulsan |
| ZIP/Postal code |
44919 |
| Country |
South Korea |
| |
|
| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
|
| Samples (4)
|
| GSM949494 |
Zebrafish retina with Id2aMM morpholino treatment, replicate 1 |
| GSM949495 |
Zebrafish retina with Id2aMM morpholino treatment, replicate 2 |
| GSM949496 |
Zebrafish retina with id2a morpholino treatment, replicate 1 |
| GSM949497 |
Zebrafish retina with id2a morpholino treatment, replicate 2 |
|
| Relations |
| BioProject |
PRJNA168635 |
| SRA |
SRP013815 |
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