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Status |
Public on May 04, 2015 |
Title |
A chromatin code for alternative splicing involving a putative association between CTCF and HP1α proteins |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Alternative splicing is primarily controlled by the activity of splicing factors and by the elongation of the RNA polymerase II (RNAPII). Recent experiments have suggested a new complex network of splicing regulation involving chromatin, transcription and multiple protein factors. In particular, the CCCTC-binding factor (CTCF), the Argonaute protein AGO1, and members of heterochromatin protein 1 (HP1) family have been implicated in the regulation of splicing associated to chromatin and the elongation of RNAPII. These results raise the question of whether these proteins may associate at the chromatin level to modulate alternative splicing.
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Overall design |
RNA extraction from MCF-7 and MCF-10A cell lines. Splicing changes between these two cell lines were measured using a splice junction array (Affymetrix HJAY).
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Contributor(s) |
Agirre E, Bellora N, Alló M, Pagès A, Bertucci P, Kornblihtt AR, Eyras E |
Citation(s) |
25934638 |
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Submission date |
Jun 21, 2012 |
Last update date |
Aug 03, 2015 |
Contact name |
Eneritz Agirre |
Organization name |
Karolinska Institutet
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Department |
MBB
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Lab |
Castelo-Branco, Molecular Neurobiology
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Street address |
Solnavägen 9
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City |
Stockholm |
ZIP/Postal code |
17165 |
Country |
Sweden |
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Platforms (1) |
GPL15106 |
[hjay] Affymetrix GeneChip Human HJAY Array |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE60094 |
A chromatin code for alternative splicing involving an association between CTCF and HP1α proteins |
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Relations |
BioProject |
PRJNA169147 |