|
| Status |
Public on Aug 18, 2012 |
| Title |
Gene expression profiling of RPMI-grown and macrophage-internalized Candida glabrata wild-type, Cgrsc3-a∆ and Cgrtt109∆ cells. |
| Platform organism |
Nakaseomyces glabratus CBS 138 |
| Sample organism |
Nakaseomyces glabratus |
| Experiment type |
Expression profiling by array
|
| Summary |
To delineate the interaction of Candida glabrata with host immune cells, we performed genome-wide transcriptional profiling analysis on THP-1 macrophage-internalized wild-type and chromatin remodeling defective mutant (Cgrsc3-a∆ and Cgrtt109∆) yeasts. Genes implicated in ergosterol biosynthesis, and high-affinity iron uptake and homeostasis were found to be down-regulated in C. glabrata wild-type and mutant cells upon macrophage internalization. Additionally, global gene expression profiles of RPMI-grown and macrophage-ingested Cgrsc3-a∆ and Cgrtt109∆ cells revealed down-regulation of genes involved in mitochondrial respiration under normal growth conditions and induction of genes required for generation of precursors of metabolites and energy upon macrophage internalization.
To examine the behavior of Candida glabrata wild-type and chromatin remodeling defective mutants upon internalization by differentiated human monocytic THP-1 cells, we compared the transcript profiles of 10 hour RPMI-grown with those of 10 hour THP-1 macrophage internalized C. glabrata cells. Additionally, early transcriptional response of C. glabrata wild-type cells to macrophage internal milieu was examined post 2 hour THP-1 macrophage infection.
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| |
|
| Overall design |
Agilent one-color experiment, Organism: Yeast (Candida glabrata)
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| |
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| Contributor(s) |
Rai MN, Balusu S, Gorityala N, Dandu L, Kaur R |
| Citation(s) |
22916016 |
| |
| Submission date |
Jun 26, 2012 |
| Last update date |
Sep 05, 2012 |
| Contact name |
Rupinder Kaur |
| E-mail(s) |
rkaur@cdfd.org.in
|
| Organization name |
Centre for DNA Fingerprinting and Diagnostics
|
| Department |
Fungal Pathogenesis
|
| Street address |
Building 7, Gruhakalpa,Nampally
|
| City |
Hyderabad |
| State/province |
AP |
| ZIP/Postal code |
500001 |
| Country |
India |
| |
|
| Platforms (1) |
| GPL15745 |
Agilent-032958 Custom Candida glabrata 4x44K Gene Expression Microarray |
|
| Samples (16)
|
| GSM952702 |
wild-type_grown in RPMI medium for 2 hours_biological replicate 1 |
| GSM952703 |
wild-type_ingested by THP-1 macrophages for 2 hours_biological replicate 1 |
| GSM952704 |
wild-type_grown in RPMI medium for 10 hours_biological replicate 1 |
| GSM952705 |
wild-type_ingested by THP-1 macrophages for 10 hours_biological replicate 1 |
| GSM952706 |
Cgrsc3-aD (CAGL0D03850g∆)_grown in RPMI medium for 10 hours_biological replicate 1 |
| GSM952707 |
Cgrsc3-aD (CAGL0D03850g∆)_ingested by THP-1 macrophages for 10 hours_biological replicate 1 |
| GSM952708 |
Cgrtt109D (CAGL0D05786g∆)_grown in RPMI medium for 10 hours_biological replicate 1 |
| GSM952709 |
Cgrtt109D (CAGL0D05786g∆)_ingested by THP-1 macrophages for 10 hours_biological replicate 1 |
| GSM952710 |
wild-type grown_in RPMI medium for 2 hours_biological replicate 2 |
| GSM952711 |
wild-type_ingested by THP-1 macrophages for 2 hours_biological replicate 2 |
| GSM952712 |
wild-type_grown in RPMI medium for 10 hours_biological replicate 2 |
| GSM952713 |
wild-type_ingested by THP-1 macrophages for 10 hours_biological replicate 2 |
| GSM952714 |
Cgrsc3-aD (CAGL0D03850g∆)_grown in RPMI medium for 10 hours_biological replicate 2 |
| GSM952715 |
Cgrsc3-aD (CAGL0D03850g∆)_ingested by THP-1 macrophages for 10 hours_biological replicate 2 |
| GSM952716 |
Cgrtt109D (CAGL0D05786g∆)_grown in RPMI medium for 10 hours_biological replicate 2 |
| GSM952717 |
Cgrtt109D (CAGL0D05786g∆)_ingested by THP-1 macrophages for 10 hours_biological replicate 2 |
|
| Relations |
| BioProject |
PRJNA169411 |
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