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Status |
Public on Feb 04, 2013 |
Title |
Expression data from developing mouse livers |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Roles of mesothelial cells (MCs) are poorly understood during liver development and injury. We identified podoplanin (Pdpn) as a cell surface markers for mesothelial cells in E12.5 mouse developing liver. To identify genes uniquely expressed in MCs, we isolated MCs from E12.5 mouse livers by FACS using anti-Pdpn antibodies and performed microarray analysis.
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Overall design |
The E12.5 liver was digested with trypsin-EDTA and incubated with antibodies against Pdpn. MCs were isolated as Pdpn+ population by FACS. Total RNA was extracted with RNAqueous Micro (Ambion) and the probes for the microarray were synthesized using the Ovation RNA amplification system V2 and FL-Ovation cDNA Biotin module V2 (Nugen). The labeled probes were hybridized with GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) and signals were analyzed with Genomic Suite software (Partek).
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Contributor(s) |
Asahina K |
Citation(s) |
23345421 |
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Submission date |
Jul 03, 2012 |
Last update date |
Feb 11, 2019 |
Contact name |
Kinji Asahina |
E-mail(s) |
asahina@usc.edu
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Organization name |
University of Southern California
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Department |
Pathology
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Street address |
1333 San Pablo Street
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City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90089-9141 |
Country |
USA |
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Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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Samples (2) |
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Relations |
BioProject |
PRJNA169864 |