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Series GSE39064 Query DataSets for GSE39064
Status Public on Feb 04, 2013
Title Expression data from developing mouse livers
Organism Mus musculus
Experiment type Expression profiling by array
Summary Roles of mesothelial cells (MCs) are poorly understood during liver development and injury. We identified podoplanin (Pdpn) as a cell surface markers for mesothelial cells in E12.5 mouse developing liver.
To identify genes uniquely expressed in MCs, we isolated MCs from E12.5 mouse livers by FACS using anti-Pdpn antibodies and performed microarray analysis.
 
Overall design The E12.5 liver was digested with trypsin-EDTA and incubated with antibodies against Pdpn. MCs were isolated as Pdpn+ population by FACS. Total RNA was extracted with RNAqueous Micro (Ambion) and the probes for the microarray were synthesized using the Ovation RNA amplification system V2 and FL-Ovation cDNA Biotin module V2 (Nugen). The labeled probes were hybridized with GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) and signals were analyzed with Genomic Suite software (Partek).
 
Contributor(s) Asahina K
Citation(s) 23345421
Submission date Jul 03, 2012
Last update date Feb 11, 2019
Contact name Kinji Asahina
E-mail(s) asahina@usc.edu
Organization name University of Southern California
Department Pathology
Street address 1333 San Pablo Street
City Los Angeles
State/province CA
ZIP/Postal code 90089-9141
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (2)
GSM954975 E12.5 mouse MCs
GSM954976 E12.5 mouse liver cells
Relations
BioProject PRJNA169864

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE39064_RAW.tar 7.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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