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| Status |
Public on Feb 25, 2013 |
| Title |
The rcf1-1 mutation affects global gene expression in Arabidopsis thaliana with or without cold stress. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Cold stress is one of the most severe environmental conditions which cause huge losses in crop production worldwide. We identified an essential regulator of cold-responsive genes and used the Affymetrix whole-genome arrays to define downstream targets of this important protein.
We used the microarrays to reveal the effect of rcf1-1 mutation on global gene expression with or without cold stress at 4°C. A set of genes differentially expressed in rcf1-1 with or without cold stress are identified.
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| Overall design |
Fourteen-day-old seedlings of Arabidopsis thaliana wild type (Columbia gl1 expressing CBF2::LUC transgene) and rcf1-1 mutant seedlings subjected to cold stress at 4°C for 0, 12, and 24 h were used for total RNA extraction and hybridizations with Affymetrix ATH1 GeneChips. There are two biological replicate per genotype.
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| |
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| Contributor(s) |
Guan Q, Wu J, Zhang Y, Jiang C, Chai C, Liu R, Zhu J |
| Citation(s) |
23371945 |
| Submission date |
Jul 03, 2012 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Jianhua Zhu |
| Phone |
3014050920
|
| Organization name |
University of Maryland
|
| Department |
Plant Science and Landscape Architecture
|
| Street address |
2102 Plant Sciences Building
|
| City |
College Park |
| State/province |
MD |
| ZIP/Postal code |
20742 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (12)
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA169923 |
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