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| Status |
Public on Jul 06, 2012 |
| Title |
A genetic basis for a postmeiotic X vs. Y chromosome intragenomic conflict in the mouse |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The Y-encoded multicopy gene Sly has been shown to repress X and Y genes in spermatids, among which are its X-linked homologs Slx and Slxl1, via modulation of post-meiotic sex chromain (PMSC). In this experiment, we compared expression in spermatids from mice that are deficient for Sly (shSLY), for Slx/Slxl1 (shSLX1) or both (shSLX1shSLY). We observed that Slx/Slxl1 has an opposite role to that of Sly, and stimulates expression of X and Y genes in spermatids when Sly is absent. Slx/Slxl1 deficiency rescues the sperm differentiation defects and near sterility caused by Sly deficiency and vice versa. Slx/Slxl1 deficiency also causes a sex ratio distortion towards the production of male offspring that is corrected by Sly deficiency.
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| Overall design |
Wild type strain-matched spermatids are used as a control (4 biological replicates), and 3 biological replicate samples are analysed for each genotype of interest (shSLY, shSLX1 and shSLX1shSLY)
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| Contributor(s) |
Cocquet J, Ellis PJ, Mahadevaiah SK, Affara NA, Vaiman D, Burgoyne PS |
| Citation(s) |
23028340 |
| |
| Submission date |
Jul 04, 2012 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Peter James Ellis |
| E-mail(s) |
p.j.i.ellis@kent.ac.uk
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| Phone |
07808167834
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| Organization name |
University of Kent
|
| Department |
School of Biosciences
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| Street address |
Stacey Building
|
| City |
Canterbury |
| State/province |
Kent |
| ZIP/Postal code |
CT2 7NJ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA170025 |
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