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Status |
Public on Jul 07, 2013 |
Title |
The rsa1-1 mutation affects global gene expression in Arabidopsis thaliana with or without salt stress |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by array
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Summary |
Salt stress is one of the most severe environmental conditions which cause huge losses in crop production worldwide. We identified a novel calcium-binding protein and used the Affymetrix whole-genome arrays to define downstream targets of this important protein. We used the microarrays to reveal the effect of rsa1-1 mutation on global gene expression in response to 120 mM Nacl for 0 or 24 h. A set of genes differentially expressed in rsa1-1 with or without salt stress are identified.
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Overall design |
Six-day-old seedlings of Arabidopsis thaliana wild type (Columbia gl1 expressing RD29A::LUC transgene) and rsa1-1 mutant seedlings subjected to salt stress with 120 mM NaCl for 0, or 24 h were used for total RNA extraction and hybridizations with Affymetrix ATH1 GeneChips. There are three biological replicate per genotype.
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Contributor(s) |
Guan Q, Wu J, Yue X, Zhang Y, Zhu J |
Citation(s) |
24009530 |
Submission date |
Jul 10, 2012 |
Last update date |
Aug 15, 2018 |
Contact name |
Jianhua Zhu |
Phone |
3014050920
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Organization name |
University of Maryland
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Department |
Plant Science and Landscape Architecture
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Street address |
2102 Plant Sciences Building
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City |
College Park |
State/province |
MD |
ZIP/Postal code |
20742 |
Country |
USA |
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Platforms (1) |
GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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Samples (12)
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Relations |
Affiliated with |
GSE69995 |
BioProject |
PRJNA170258 |