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| Status |
Public on Jan 04, 2013 |
| Title |
Microarray analysis of purified germ cells in mouse |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Microarray analysis of purified pachytene spermatocytes and round spermatids. Each stage was examined in wild type and RNF8 knockout mice in two biological replicates.
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| Overall design |
We performed microarray analysis using Affymetrix Gene 1.0 ST Arrays with purified pachytene spermatocytes and round spermatids.
Pachytene spermatocytes and round spermatids were enriched from 3 to 4 males from the WT or Rnf8-KO via BSA gravity sedimentation according to the previous publication [PMID 8231890] and >95% (PS, RS) enrichments were verified after DAPI staining under a fluorescent microscope. For microarray analysis, total RNAs from purified pachytene spermatocytes or round spermatids were examined.
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| Contributor(s) |
Sin H, Namekawa S |
| Citation(s) |
23249736 |
| |
| Submission date |
Jul 12, 2012 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Satoshi Namekawa |
| E-mail(s) |
satoshi.namekawa@cchmc.org
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| Phone |
513-803-1377
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| Organization name |
Cincinnati Children's Hospital Medical Center
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| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA170524 |
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