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| Status |
Public on Jan 02, 2013 |
| Title |
Genome-Wide Characterization of the Phosphate Starvation Response in Schizosaccharomyces pombe [ChIP-Seq data] |
| Organism |
Schizosaccharomyces pombe 972h- |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Inorganic phosphate is an essential nutrient required by organisms for growth. During phosphate starvation, Saccharomyces cerevisiae activates the phosphate signal transduction (PHO) pathway leading to the expression of the secreted acid phosphatase, PHO5. The fission yeast, Schizosaccharomyces pombe, regulates expression of the ScPHO5 homolog (pho1+) via a non-orthologous PHO pathway. The genes induced by phosphate limitation and the molecular mechanism by which the genetically identified positive (pho7+) and negative (csk1+) regulators function are not known. Here we use a combination of molecular biology, expression microarrays, and chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-Seq) to characterize the role of pho7+ and csk1+ in the PHO response. We define the set of genes that comprise the initial response to phosphate starvation in S. pombe. We identify a conserved PHO response for the ScPHO5 (pho1+), ScPHO84 (spbc8e4.01c+), and ScGIT1 (spbc1271.09+) orthologs. We use ChIP-Seq to identify members of the Pho7 regulon and characterize Pho7 binding in response to phosphate-limitation and Csk1 activity. We demonstrate that activation of pho1+ requires Pho7 binding to a UAS in the pho1+ promoter and that Csk1 repression does not regulate Pho7 enrichment. Further, we find that Pho7-dependent activation is not limited to phosphate-starvation, as additional environmental stress response pathways require pho7+ for maximal induction. We provide a global analysis of the PHO pathway in S. pombe. Our results elucidate the conserved core regulon required for responding to phosphate starvation between distantly related ascomycetes and a better understanding of flexibility in environmental stress response networks.
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| |
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| Overall design |
ChIP Sequencing of the Schizosaccharomyces pombe 972h- transcription factor Pho7-TAP in high-Pi, no-Pi, and csk1Δ conditions
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| |
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| Contributor(s) |
Carter-O'Connell IO, Peel MT, Wykoff DD, O'Shea EK |
| Citation(s) |
23231582 |
| |
| Submission date |
Jul 19, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Ian O'Brien Carter-O'Connell |
| Organization name |
Harvard University
|
| Department |
MCB
|
| Lab |
O'Shea
|
| Street address |
52 Oxford St. NW 440
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15830 |
Illumina HiSeq 2000 (Schizosaccharomyces pombe 972h-) |
|
| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE39499 |
Genome-Wide Characterization of the Phosphate Starvation Response in Schizosaccharomyces pombe |
|
| Relations |
| SRA |
SRP014477 |
| BioProject |
PRJNA171103 |
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