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Series GSE3987 Query DataSets for GSE3987
Status Public on Feb 03, 2006
Title Combinatorial regulation of estrogen receptor alpha-responsive promoters
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary In breast cancer and normal estrogen target tissues, estrogen receptor alpha (ERalpha) signaling results in establishment of spatiotemporal patterns of gene expression. A time-course series of ChIP-chip experiments were performed to identify direct ERalpha target genes and determine whether these targets were transcriptionally activated or repressed by ERalpha.
Keywords: Time-course ChIP-chip
 
Overall design A total of 24 samples were analyzed. MCF7 cells were treated with E2 for 0 (control), 3, 12 and 24 hours. ChIP assays were performed using antibodies against ERalpha and specific histone modifications (acetyl-H3-K9 and dimethyl-H3-K9). The immunoprecipitated DNA was used to probe the 12K CGI microarray. Two independent experiments were performed for each time-point treatment and antibody.
 
Contributor(s) Liyanarachchi S
Citation(s) 16455494
Submission date Jan 06, 2006
Last update date Mar 16, 2012
Contact name Sandya Liyanarachchi
Organization name OSU
Department Human Genetics
Lab de la Chapelle Lab
Street address Rm 830, 460, W 12th Avenue
City Columbus
State/province OH
ZIP/Postal code 43210
Country USA
 
Platforms (1)
GPL2040 UHN Human CpG 12K Array (HCGI12K)
Samples (24)
GSM91087 MCF7 cells treated with estradiol for 0hr, immunoprecipitated with ERa antibody, replicate 1
GSM91088 MCF7 cells treated with estradiol for 0hr, immunoprecipitated with ERa antibody, replicate 2
GSM91089 MCF7 cells treated with estradiol for 3hr, immunoprecipitated with ERa antibody, replicate 1
Relations
BioProject PRJNA94257

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE3987_RAW.tar 25.3 Mb (http)(custom) TAR (of GPR)

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