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Status |
Public on Feb 03, 2006 |
Title |
Combinatorial regulation of estrogen receptor alpha-responsive promoters |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by genome tiling array
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Summary |
In breast cancer and normal estrogen target tissues, estrogen receptor alpha (ERalpha) signaling results in establishment of spatiotemporal patterns of gene expression. A time-course series of ChIP-chip experiments were performed to identify direct ERalpha target genes and determine whether these targets were transcriptionally activated or repressed by ERalpha. Keywords: Time-course ChIP-chip
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Overall design |
A total of 24 samples were analyzed. MCF7 cells were treated with E2 for 0 (control), 3, 12 and 24 hours. ChIP assays were performed using antibodies against ERalpha and specific histone modifications (acetyl-H3-K9 and dimethyl-H3-K9). The immunoprecipitated DNA was used to probe the 12K CGI microarray. Two independent experiments were performed for each time-point treatment and antibody.
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Contributor(s) |
Liyanarachchi S |
Citation(s) |
16455494 |
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Submission date |
Jan 06, 2006 |
Last update date |
Mar 16, 2012 |
Contact name |
Sandya Liyanarachchi |
Organization name |
OSU
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Department |
Human Genetics
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Lab |
de la Chapelle Lab
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Street address |
Rm 830, 460, W 12th Avenue
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City |
Columbus |
State/province |
OH |
ZIP/Postal code |
43210 |
Country |
USA |
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Platforms (1) |
GPL2040 |
UHN Human CpG 12K Array (HCGI12K) |
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Samples (24)
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GSM91087 |
MCF7 cells treated with estradiol for 0hr, immunoprecipitated with ERa antibody, replicate 1 |
GSM91088 |
MCF7 cells treated with estradiol for 0hr, immunoprecipitated with ERa antibody, replicate 2 |
GSM91089 |
MCF7 cells treated with estradiol for 3hr, immunoprecipitated with ERa antibody, replicate 1 |
GSM91090 |
MCF7 cells treated with estradiol for 3hr, immunoprecipitated with ERa antibody, replicate 2 |
GSM91091 |
MCF7 cells treated with estradiol for 12hr, immunoprecipitated with ERa antibody, replicate 1 |
GSM91092 |
MCF7 cells treated with estradiol for 12hr, immunoprecipitated with ERa antibody, replicate 2 |
GSM91093 |
MCF7 cells treated with estradiol for 24hr, immunoprecipitated with ERa antibody, replicate 1 |
GSM91094 |
MCF7 cells treated with estradiol for 24hr, immunoprecipitated with ERa antibody, replicate 2 |
GSM91095 |
MCF7 cells treated with estradiol for 0hr, immunoprecipitated with Ac-H3-K9 antibody, replicate 1 |
GSM91096 |
MCF7 cells treated with estradiol for 0hr, immunoprecipitated with Ac-H3-K9 antibody, replicate 2 |
GSM91097 |
MCF7 cells treated with estradiol for 3hr, immunoprecipitated with Ac-H3-K9 antibody, replicate 1 |
GSM91098 |
MCF7 cells treated with estradiol for 3hr, immunoprecipitated with Ac-H3-K9 antibody, replicate 2 |
GSM91099 |
MCF7 cells treated with estradiol for 12hr, immunoprecipitated with Ac-H3-K9 antibody, replicate 1 |
GSM91100 |
MCF7 cells treated with estradiol for 12hr, immunoprecipitated with Ac-H3-K9 antibody, replicate 2 |
GSM91101 |
MCF7 cells treated with estradiol for 24hr, immunoprecipitated with Ac-H3-K9 antibody, replicate 1 |
GSM91102 |
MCF7 cells treated with estradiol for 24hr, immunoprecipitated with Ac-H3-K9 antibody, replicate 2 |
GSM91103 |
MCF7 cells treated with estradiol for 0hr, immunoprecipitated with diMe-H3-K9 antibody, replicate 1 |
GSM91104 |
MCF7 cells treated with estradiol for 0hr, immunoprecipitated with diMe-H3-K9 antibody, replicate 2 |
GSM91105 |
MCF7 cells treated with estradiol for 3hr, immunoprecipitated with diMe-H3-K9 antibody, replicate 1 |
GSM91106 |
MCF7 cells treated with estradiol for 3hr, immunoprecipitated with diMe-H3-K9 antibody, replicate 2 |
GSM91107 |
MCF7 cells treated with estradiol for 12hr, immunoprecipitated with diMe-H3-K9 antibody, replicate 1 |
GSM91108 |
MCF7 cells treated with estradiol for 12hr, immunoprecipitated with diMe-H3-K9 antibody, replicate 2 |
GSM91109 |
MCF7 cells treated with estradiol for 24hr, immunoprecipitated with diMe-H3-K9 antibody, replicate 1 |
GSM91110 |
MCF7 cells treated with estradiol for 24hr, immunoprecipitated with diMe-H3-K9 antibody, replicate 2 |
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Relations |
BioProject |
PRJNA94257 |
Supplementary file |
Size |
Download |
File type/resource |
GSE3987_RAW.tar |
25.3 Mb |
(http)(custom) |
TAR (of GPR) |
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