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| Status |
Public on May 10, 2006 |
| Title |
Methylation profiling of leukemia cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Methylation of CpG islands is associated with transcriptional repression and, in cancer, leads to the abnormal silencing of tumor-suppressor genes. We developed a novel and robust technique that allows the unbiased, genome wide detection of CpG-methylation in limited DNA samples, without applying methylation-sensitive restriction endonucleases or bisulfite-treatment. The approach is based on a recombinant, methyl-CpG binding protein that efficiently binds CpG-methylated DNA depending on its degree of CpG methylation. Its application in methyl-CpG immunoprecipitation (MCIp) facilitates the monitoring of CpG-island methylation on a genome wide level (in combination with CpG-island microarrays). The power of this novel approach was demonstrated by the profiling of three myeloid cell lines leading to the identification of more than a hundred aberrantly methylated CpG islands and many novel, putative tumor-suppressor genes.
Keywords: MCIp on Chip
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| Overall design |
The methylation profiles of the three leukemia cell lines KG-1, U937 and THP-1 were compared to normal human blood monocytes (reference). The set includes three independent hybridizations for each sample.
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| Contributor(s) |
Gebhard C, Schwarzfischer L, Rehli M |
| Citation(s) |
16778185 |
| Submission date |
Jan 10, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Michael Rehli |
| E-mail(s) |
michael.rehli@klinik.uni-r.de
|
| Organization name |
University Hospital Regensburg
|
| Department |
Internal Med III
|
| Street address |
F.-J.-Strauss-Allee 11
|
| City |
Regensburg |
| ZIP/Postal code |
93042 |
| Country |
Germany |
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| Platforms (1) |
| GPL2040 |
UHN Human CpG 12K Array (HCGI12K) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA95231 |
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