|
| Status |
Public on Aug 16, 2012 |
| Title |
Comparative transcription profiling of heritage maize lines |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by array
|
| Summary |
Three different maize lines were assayed for differential gene expression in mature leaf tissue. Leaves from the Oh43 maize line are more resistant to insect larvae damage than the original parents, lines Oh40B and W8. The goal of the project was to discover genes highly expressed in the Oh43 line that potentially contributes to insect resistance.
|
| |
|
| Overall design |
RNA was extracted from a mature leaf (third visible leaf from the top of a 7 leaf plant) of each inbred (Oh43, Oh40B, and W8). cDNA from four biological replicates of each inbred was labeled (indirect method) twice with Cy5 and twice with Cy3. A total of six microarray slides were probed with 12 different labeled cDNAs.
|
| |
|
| Contributor(s) |
Johnson ET, Dowd PF |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 14, 2012 |
| Last update date |
Sep 12, 2012 |
| Contact name |
Eric Johnson |
| E-mail(s) |
eric.johnson2@ars.usda.gov
|
| Phone |
309-681-6177
|
| Organization name |
USDA ARS
|
| Department |
Crop Bioprotection Research
|
| Street address |
1815 N. University St.
|
| City |
Peoria |
| State/province |
IL |
| ZIP/Postal code |
61604 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6438 |
Maize oligonucleotide array 46K version |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA172925 |
Less...
More...