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Status |
Public on Aug 01, 2017 |
Title |
Transcriptome analysis of tomato in response to treatment with neutralized phosphorous acid (NPA) |
Organism |
Solanum lycopersicum |
Experiment type |
Expression profiling by array
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Summary |
Phosphonate related fungicides such as neutralized phosphorous acid (NPA) are effective for the control of plant diseases, especially those caused by Phytophthora and other oomycetes. It has been suggested that phosphonate may induce plant resistance, however, the mechanism is not clear thus far. We performed microarray analysis using Affymetrix Tomato Genome Array to identify tomato genes that are differentially expressed in response to NPA treatment.
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Overall design |
At 6 and 12 hours post treatment (hpt) of the tomato plants with NPA or water (ddH2O, as the control), total RNAs were isolated from leaves and subjected to microarray analysis by the use of Affymetrix Tomato Genome Array. To reduce the biological variability, each hybridization experiment was performed with RNA prepared from pools of five plants. The total set of experiments was repeated twice.
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Contributor(s) |
Lin CP, Ann PJ, Liou RF |
Citation missing |
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Submission date |
Aug 16, 2012 |
Last update date |
Aug 01, 2017 |
Contact name |
Ruey-Fen Liou |
E-mail(s) |
rfliou@ntu.edu.tw
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Organization name |
National Taiwan University
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Department |
Plant Pathology and Microbiology
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Street address |
1, Sec. 4, Roosevelt Road
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City |
Taipei |
ZIP/Postal code |
106 |
Country |
Taiwan |
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Platforms (1) |
GPL4741 |
[Tomato] Affymetrix Tomato Genome Array |
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Samples (8)
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GSM986571 |
tomato leaves at 6 hpt with water, biological rep1 |
GSM986572 |
tomato leaves at 6 hpt with water, biological rep2 |
GSM986573 |
tomato leaves at 6 hpt with NPA, biological rep1 |
GSM986574 |
tomato leaves at 6 hpt with NPA, biological rep2 |
GSM986575 |
tomato leaves at 12 hpt with water, biological rep1 |
GSM986576 |
tomato leaves at 12 hpt with water, biological rep2 |
GSM986577 |
tomato leaves at 12 hpt with NPA, biological rep1 |
GSM986578 |
tomato leaves at 12 hpt with NPA, biological rep2 |
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This SubSeries is part of SuperSeries: |
GSE40214 |
Transcriptome analysis of neutralized phosphorous acid (NPA)-induced resistance in tomato against Phytophthora parasitica |
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Relations |
BioProject |
PRJNA173208 |