 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 01, 2017 |
| Title |
Transcriptome analysis of phosphonate-pretreated tomato in response to inoculation with Phytophthora parasitica |
| Organism |
Solanum lycopersicum |
| Experiment type |
Expression profiling by array
|
| Summary |
Phosphonate related fungicides such as neutralized phosphorous acid (NPA) are effective for the control of plant diseases caused by Oomycetes including Phytophthora parasitica. It has been proposed that phosphonate may induce plant resistance. However, the mechanism underlying phosphonate-induced resistance remains unclear.
The purpose of this study is to identify genes that are differentially expressed in phosphonate-pretreated tomato plants in response to inoculation with Phytophthora parasitica.
|
| |
|
| Overall design |
We treated the tomato plants with NPA or water (ddH2O, as a control) for three days, and then inoculated the plants with zoospores of P. parasitica. For microarray analysis by the use of Affymetrix Tomato Genome Array, total RNAs were isolated from plant leaves at: (1) 3 days post treatment (dpt) with NPA or water, (2) 6 hours post inoculation (hpi) of the NPA- or water-pretreated plants, (3) 12 hpi of the NPA- or water-pretreated plants. To reduce the biological variability, each hybridization experiment was performed with RNA prepared from pools of five plants. The total set of experiments was repeated three times.
|
| |
|
| Contributor(s) |
Lin CP, Ann PJ, Liou RF |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 16, 2012 |
| Last update date |
Aug 01, 2017 |
| Contact name |
Ruey-Fen Liou |
| E-mail(s) |
rfliou@ntu.edu.tw
|
| Organization name |
National Taiwan University
|
| Department |
Plant Pathology and Microbiology
|
| Street address |
1, Sec. 4, Roosevelt Road
|
| City |
Taipei |
| ZIP/Postal code |
106 |
| Country |
Taiwan |
| |
|
| Platforms (1) |
| GPL4741 |
[Tomato] Affymetrix Tomato Genome Array |
|
| Samples (18)
|
| GSM986610 |
tomato leaves at 3 dpt with water, biological rep1 |
| GSM986611 |
tomato leaves at 3 dpt with water, biological rep2 |
| GSM986612 |
tomato leaves at 3 dpt with water, biological rep3 |
| GSM986613 |
tomato leaves at 3 dpt with NPA, biological rep1 |
| GSM986614 |
tomato leaves at 3 dpt with NPA, biological rep2 |
| GSM986617 |
tomato leaves at 3 dpt with NPA, biological rep3 |
| GSM986618 |
tomato leaves at 6 hpi of water-pretreated plants, biological rep1 |
| GSM986621 |
tomato leaves at 6 hpi of water-pretreated plants, biological rep2 |
| GSM986623 |
tomato leaves at 6 hpi of water-pretreated plants, biological rep3 |
| GSM986625 |
tomato leaves at 6 hpi of NPA-pretreated plants, biological rep1 |
| GSM986627 |
tomato leaves at 6 hpi of NPA-pretreated plants, biological rep2 |
| GSM986629 |
tomato leaves at 6 hpi of NPA-pretreated plants, biological rep3 |
| GSM986631 |
tomato leaves at 12 hpi of water-pretreated plants, biological rep1 |
| GSM986633 |
tomato leaves at 12 hpi of water-pretreated plants, biological rep2 |
| GSM986634 |
tomato leaves at 12 hpi of water-pretreated plants, biological rep3 |
| GSM986636 |
tomato leaves at 12 hpi of NPA-pretreated plants, biological rep1 |
| GSM986638 |
tomato leaves at 12 hpi of NPA-pretreated plants, biological rep2 |
| GSM986640 |
tomato leaves at 12 hpi of NPA-pretreated plants, biological rep3 |
|
| This SubSeries is part of SuperSeries: |
| GSE40214 |
Transcriptome analysis of neutralized phosphorous acid (NPA)-induced resistance in tomato against Phytophthora parasitica |
|
| Relations |
| BioProject |
PRJNA173209 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40164_RAW.tar |
17.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...