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| Status |
Public on Jan 01, 2013 |
| Title |
Transcriptomic analysis of differential genetic expression under CosR-knockdown condition compared with wild-type |
| Organism |
Campylobacter jejuni |
| Experiment type |
Expression profiling by array
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| Summary |
We perfomed the transcriptomic analysis of differential genetic expression in the CosR-knockdown condition with a DNA microarray. CosR-specific antisense-PNA was used for CosR-knockdown. The results revealed that CosR significantly affected the expression of several genes involved in various cellular function in Campylobacter. Final goal of this study is to figure out the importance and the role of CosR in Campylobacter by identification of the CosR regulons.
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| Overall design |
Two-condition experiment, WT vs. CosR-knockdown. Biological replicates: 3 wild type (control) replicates, 3 CosR-knockdown replicates. For preparing the total RNA, C. jejuni cells were grown at 42°C in MH broth supplemented with 1.5 μM CosR-PNA for CosR knockdown to the mid-exponential phase for approximately 8 hr with shaking.
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| Contributor(s) |
Hwang S, Zhang Q, Ryu S, Jeon B |
| Citation(s) |
23065977 |
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| Submission date |
Aug 17, 2012 |
| Last update date |
Jan 07, 2013 |
| Contact name |
Sunyoung Hwang |
| E-mail(s) |
skyborn7@snu.ac.kr
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| Organization name |
Seoul National University
|
| Department |
Department of Food and Animal Biotechnology
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| Lab |
Lab of Molecular Food Microbiology
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| Street address |
599 Gwanak-ro
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| City |
Seoul |
| ZIP/Postal code |
151921 |
| Country |
South Korea |
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| Platforms (1) |
| GPL15955 |
Campylobacter jejuni subsp. jejuni NCTC 11168 15K Microarrays |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA173090 |
