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| Status |
Public on Apr 05, 2013 |
| Title |
Identification of glucose-TOR signaling early target genes in Arabidopsis seedling autotrophic transition stage |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
The goal of this experiment was to explore the molecular network of glucose-TOR signaling in Arabidopsis seedling autotrophic transition stage. We used the whole-genome microarrays to detail the global program of gene expression mediated by glucose and TOR.
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| Overall design |
Arabidopsis WT and estradiol inducible RNAi-tor seedlings were germinated in low light condition for 3 days to deplete seed nutrient supply. Fifteen mM glucose was adding back to reactivate the quiescent seedlings and the samples were harvested for RNA isolation. Transcript profiles were analyzed and compared between with and without glucose treatment to identify early glucose-TOR target genes. Each experimental condition was repeated three times and total 12 samples were generated.
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| Contributor(s) |
Xiong Y, Xiang C, McCormack M, Sheen J |
| Citation(s) |
23542588 |
| Submission date |
Aug 21, 2012 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Jen Sheen |
| E-mail(s) |
sheen@molbio.mgh.harvard.edu
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Molecular Biology
|
| Lab |
Sheen
|
| Street address |
185 Cambridge st. CPZN7620
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114-2790 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (12)
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| GSM989193 |
wild type (Col) without treatment, biological rep1 |
| GSM989194 |
wild type (Col) without treatment, biological rep2 |
| GSM989195 |
wild type (Col) without treatment, biological rep3 |
| GSM989196 |
wild type (Col) glucose treatment, biological rep1 |
| GSM989197 |
wild type (Col) glucose treatment, biological rep2 |
| GSM989198 |
wild type (Col) glucose treatment, biological rep3 |
| GSM989199 |
tor mutant without treatment, biological rep1 |
| GSM989200 |
tor mutant without treatment, biological rep2 |
| GSM989201 |
tor mutant without treatment, biological rep3 |
| GSM989202 |
tor mutant with glucose treatment, biological rep1 |
| GSM989203 |
tor mutant with glucose treatment, biological rep2 |
| GSM989204 |
tor mutant with glucose treatment, biological rep3 |
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA173332 |
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