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Status |
Public on Jan 13, 2006 |
Title |
Rapid selection response for contextual fear conditioning in a cross between C57BL/6J and A/J (palme-affy-mouse-198967) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
These data are from the brains (amygdala and hippocampus) of mice originally derived from a cross between C57BL/6J and A/J inbred strains. We used short-term selection to produce outbred mouse lines with differences in contextual fear conditioning, which is a measure of fear learning. We selected for a total of 4 generations. Fear learning differed in the selected lines and this difference was stronger with each successive generation of selection. We identified several QTLs for the selection response, including a highly significant QTL at the tyr locus (p < 9.6(-10)). We used Affymetrix microarrays to identify many differentially expressed genes in the amygdala and hippocampus of mice from the final generation of selection. Amygdala and hippocampus samples were rapidly dissected out of experimentally naïve mice from each selected line. Three samples were pooled and hybridized to each array. Experimentally naïve mice were used because the behavior of the mice can be reliably a nticipated due to their lineage. Thus these gene expression differences are not due to the response to human handling, foot shock or fear-inducing conditioned stimuli. We have a second similar study that focuses on a different selected population that was based on C57BL/6J and DBA/2J mice (see GES4035).
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Contributor(s) |
Palmer AA |
Citation(s) |
18363093 |
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Submission date |
Jan 13, 2006 |
Last update date |
Feb 11, 2019 |
Contact name |
Winnie Liang |
E-mail(s) |
wliang@tgen.org
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Organization name |
Translational Genomics
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Street address |
445 N. Fifth Street
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City |
Phoenix |
State/province |
AZ |
ZIP/Postal code |
85012 |
Country |
USA |
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Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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Samples (24)
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GSM92409 |
brain, hippocampus: Hippocampus 1_le1 |
GSM92410 |
brain, hippocampus: Hippocampus 2_le1 |
GSM92411 |
brain, hippocampus: Hippocampus 3_le1 |
GSM92412 |
brain, hippocampus: Hippocampus 4_le1 |
GSM92413 |
brain, hippocampus: Hippocampus 5_le1 |
GSM92414 |
brain, hippocampus: Hippocampus 6_le1 |
GSM92415 |
brain, hippocampus: Hippocampus 7_le1 |
GSM92416 |
brain, hippocampus: Hippocampus 8_le1 |
GSM92417 |
brain, hippocampus: Hippocampus 9_le1 |
GSM92418 |
brain, hippocampus: Hippocampus 10_le1 |
GSM92419 |
brain, hippocampus: Hippocampus 11_le1 |
GSM92420 |
brain, hippocampus: Hippocampus 12_le1 |
GSM92421 |
brain, amygdala: Amygdala 1_le1 |
GSM92422 |
brain, amygdala: Amygdala 2_le1 |
GSM92423 |
brain, amygdala: Amygdala 3_le1 |
GSM92424 |
brain, amygdala: Amygdala 4_le1 |
GSM92425 |
brain, amygdala: Amygdala 5_le1 |
GSM92426 |
brain, amygdala: Amygdala 6_le1 |
GSM92427 |
brain, amygdala: Amygdala 7_le1 |
GSM92428 |
brain, amygdala: Amygdala 8_le1 |
GSM92429 |
brain, amygdala: Amygdala 9_le1 |
GSM92430 |
brain, amygdala: Amygdala 10_le1 |
GSM92432 |
brain, amygdala: Amygdala 11_le1 |
GSM92433 |
brain, amygdala: Amygdala 12_le1 |
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Relations |
BioProject |
PRJNA95159 |
Supplementary file |
Size |
Download |
File type/resource |
GSE4034_RAW.tar |
77.4 Mb |
(http)(custom) |
TAR (of CEL) |
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