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| Status |
Public on Aug 28, 2012 |
| Title |
Identification of a specific reprogramming-associated epigenetic signature in human induced pluripotent stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
In this work, we characterized the epigenomic integrity of 17 hiPSC lines derived from six different cell types with varied reprogramming efficiencies. We demonstrate that epigenetic aberrations are a general feature of the hiPSC state and are independent of the somatic cell source. Additionally, we determine that both shared and line-specific epigenetic aberrations in hiPSCs can directly translate into changes in gene expression in both the pluripotent and differentiated states.
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| |
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| Overall design |
Examination of differential methylation between iPSC and ESCs through differentiated states.
Examination of differential methylation between iPSC and somatic cell source and iPSC and ESCs.
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| |
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| Contributor(s) |
Plongthongkum N, Diep DH, Gore A, Fung H, Zhang K |
| Citation(s) |
22991473 |
| |
| Submission date |
Aug 24, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Kun Zhang |
| E-mail(s) |
k4zhang@ucsd.edu
|
| Organization name |
University of California, San Diego
|
| Department |
Bioengineering
|
| Lab |
Integrative Genomics Laboratory
|
| Street address |
9500 Gilman Dr Mailcode: 0412
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (65)
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| Relations |
| SRA |
SRP015122 |
| BioProject |
PRJNA173887 |
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