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| Status |
Public on Aug 31, 2012 |
| Title |
Expression data from normal human fibroblasts expressing prelamin A or progerin, untreated or treated with farnesyltransferase inhibitor (FTI) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We compared the transcriptomes of isogenic diploid fibroblasts expressing progerin or elevated levels of wild-type prelamin A with that of wild-type fibroblasts. We subsequently used the reversion towards normal of two phenotypes, reduced cell growth and dismorphic nuclei, by treatment with farnesyltransferase inhibitor (FTI) or overexpression of ZMPSTE24, as a filtering strategy to identify genes linked to the onset of these two phenotypes.
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| Overall design |
We carried out microarray analyses of gene expression profiling in isogenic fibroblasts lines to identify the subset of genes whose expression patterns are strongly altered upon expression of progerin or elevated levels of wild-type lamin A. A filtering strategy was then used to identify potential key effectors. We searched for genes whose expression was reverted towards normal by treatment with farnesyl transferase inibitors (FTI) in both cell lines for 48 hours, or by overexpression of ZMPSTE24 in cells with elevated levels of prelamin A, conditions that improve cell proliferation and leads to a significant decrease in nuclear membrane abnormalities.
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| Contributor(s) |
Candelario J, Comai L |
| Citation(s) |
22948034 |
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| Submission date |
Aug 27, 2012 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Lucio Comai |
| E-mail(s) |
comai@usc.edu
|
| Organization name |
University of Southern California
|
| Street address |
2250 Alcazar Street
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| City |
Los Angeles |
| ZIP/Postal code |
90033 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA173883 |
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