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| Status |
Public on Sep 08, 2012 |
| Title |
Transcriptional response of Escherichia coli O157:H7 to cinnamaldehyde |
| Platform organism |
Escherichia coli |
| Sample organism |
Escherichia coli O157:H7 |
| Experiment type |
Expression profiling by array
|
| Summary |
Cinnamaldehyde is a natural antimicrobial and has been found to be effective against many foodborne pathogens including Escherichia coli O157:H7. Although its antimicrobial effects have been well investigated, limited information is available on its effects at the molecular level. Sublethal treatment at 200 mg/l cinnamaldehyde inhibited growth of E. coli O157:H7 at 37oC and for ≤ 2 h caused cell elongation, but from 2 to 4 h growth resumed and cells reverted to normal length. To understand this transient behaviour, genome-wide transcriptional analysis of E. coli O157:H7 was performed at 2 and 4 h exposure to cinnamaldehyde. Drastically different gene expression profiles were obtained at 2 and 4 h. At 2 h exposure, cinnamaldehyde induced overexpression of many oxidative stress-related genes, reduced DNA replication, and synthesis of protein, O-antigen and fimbriae. At 4 h, many cinnamaldehyde-induced repressive effects on E. coli O157:H7 gene expressions were reversed and oxidatve stress genes were nolonger differentially expressed.
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| Overall design |
Duplicate E. coli O157:H7 cultures with or without 200 mg/l cinnamaldehyde were incubated at 37°C for ≤ 4 h. Cinnamaldehyde-induced changes in gene expression profiles were compared at 2 and 4 h using Affymetrix Ginechip 2.0 microarrays.
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| Contributor(s) |
Visvalingam J, Hernandez-Doria JD, Holley RA |
| Citation(s) |
23183978 |
| |
| Submission date |
Sep 07, 2012 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Richard A Holley |
| E-mail(s) |
rick_holley@umanitoba.ca
|
| Fax |
0012044747630
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| Organization name |
University of Manitoba
|
| Department |
Department of Food Science
|
| Street address |
250 Ellis Building
|
| City |
Winnipeg |
| State/province |
Manitoba |
| ZIP/Postal code |
R3T 2N2 |
| Country |
Canada |
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| Platforms (1) |
| GPL3154 |
[E_coli_2] Affymetrix E. coli Genome 2.0 Array |
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| Samples (8)
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| GSM999245 |
E. coli O157:H7 at 2 h control biological rep1 |
| GSM999246 |
E. coli O157:H7 at 2 h control biological rep2 |
| GSM999247 |
E. coli O157:H7 at 4 h control biological rep1 |
| GSM999248 |
E. coli O157:H7 at 4 h control biological rep2 |
| GSM999249 |
E. coli O157:H7 at 2 h cinnamaldehyde treatment biological rep1 |
| GSM999250 |
E. coli O157:H7 at 2 h cinnamaldehyde treatment biological rep2 |
| GSM999251 |
E. coli O157:H7 at 4 h cinnamaldehyde treatment biological rep1 |
| GSM999252 |
E. coli O157:H7 at 4 h cinnamaldehyde treatment biological rep2 |
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| Relations |
| BioProject |
PRJNA174627 |
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