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Series GSE407 Query DataSets for GSE407
Status Public on Apr 15, 2003
Title Hypo-osmotic shock - ppt1 and wildtype
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Wild type and ppt1 mutant under hypo-osmotic shock.

Cultures were grown with 1M sorbitol for ~20 hours, cells were collected by centrifugation and resuspended in YPD at time zero. Samples were collected at 0, 7, 15, 30 and 60 minutes after transfer to YPD.

Experimental samples were used to generate Cy5-labeled cDNA probes, whereas mRNA reference pools extracted from cultures of the respective strains grown to early log phase under normal conditions, were used to generate Cy3-labeled cDNA probes. Cy5- and Cy3-labeled probes were hybridized together to microarrays printed with PCR-amplified fragments, representing 6280 of the Saccharomyces cerevisiae ORFs.
Keywords: time-course
 
 
Contributor(s) Segal E, Shapira M, Regev A, Pe'er D, Botstein D, Koller D, Friedman N
Citation(s) 12740579
Submission date Apr 15, 2003
Last update date Feb 23, 2012
Contact name Eran Segal
E-mail(s) eran@cs.stanford.edu
Phone 650-725-8784
URL http://cs.stanford.edu/~eran
Organization name Stanford University
Department Computer Science
Street address Gates Building 1A
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL288 Segal_2
Samples (10)
GSM6014 wt_hypo_osmotic_shock_0min
GSM6015 delPPT1_hypo_osmotic_shock_60min
GSM6016 wt_hypo_osmotic_shock_7min
Relations
BioProject PRJNA84955

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary data files not provided

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