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| Status |
Public on Feb 01, 2015 |
| Title |
Transcription factor Msn2 dependent gene expression in two entomopathogen fungi, Beauveria bassiana and Metarhizium robertsii under thermal and oxidaitve stresses |
| Organisms |
Beauveria bassiana; Metarhizium robertsii |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transcription factor Msn2 played crucial roles in mediating fungal stress tolerance, a determinant to the biocontrol potential of fungal entomopathogens. We characterized for the first time the functions of Beauveria bassiana Msn2 (BbMsn2) and Metarhizium robertsii (MrMsn2) by analyzing multi-phenotypic changes in Msn2-deletion and investigating transcription patterns of WT versus ΔMsn2 of B. bassiana and M. robertsii under thermal and oxidative stresses by using high throughput sequencing (RNA-Seq). Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which involved in transportation, detoxification, signal transduction, and energy metabolism, were significantly repressed in expression level.
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| Overall design |
Total RNA obtained from Bbmsn2 and MrMsn2 disruption mutant subjected to 2 mM menadione and 40 °C for 3-h response compared to the wild type strain under the same stress treatment.
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| Contributor(s) |
Liu Q, Feng M |
| Citation(s) |
23466345 |
| |
| Submission date |
Sep 10, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Liu Qian |
| E-mail(s) |
liu_q1@tib.cas.cn
|
| Organization name |
TIB CAS
|
| Street address |
Xi 7 Dao No.32 Airport Economic Area
|
| City |
Tianjin |
| ZIP/Postal code |
300308 |
| Country |
China |
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| Platforms (2) |
| GPL16038 |
Illumina HiSeq 2000 (Beauveria bassiana) |
| GPL16039 |
Illumina HiSeq 2000 (Metarhizium robertsii) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA174866 |
| SRA |
SRP015706 |
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