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| Status |
Public on Apr 15, 2003 |
| Title |
Stationary phase - ypl230w mutant and wildtype |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
Wild type and ypl230w mutant under stationary phase.
Cultures were grown to OD600 of 0.27 (Ypl230w mutants) and 0.4 (congenic wild-type; DBY8778), at which point time zero samples were collected. Samples were further collected at 2 (or 3), 5, 7, 9, and 24 hours.
Experimental samples were used to generate Cy5-labeled cDNA probes, whereas mRNA reference pools extracted from cultures of the respective strains grown to early log phase under normal conditions, were used to generate Cy3-labeled cDNA probes. Cy5- and Cy3-labeled probes were hybridized together to microarrays printed with PCR-amplified fragments, representing 6280 of the Saccharomyces cerevisiae ORFs.
Keywords: time-course
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| Contributor(s) |
Segal E, Shapira M, Regev A, Pe'er D, Botstein D, Koller D, Friedman N |
| Citation(s) |
12740579 |
| Submission date |
Apr 15, 2003 |
| Last update date |
Feb 23, 2012 |
| Contact name |
Eran Segal |
| E-mail(s) |
eran@cs.stanford.edu
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| Phone |
650-725-8784
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| URL |
http://cs.stanford.edu/~eran
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| Organization name |
Stanford University
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| Department |
Computer Science
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| Street address |
Gates Building 1A
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| GSM6024 |
DBY8778(wt)_stationary_phase_0hours |
| GSM6025 |
delUSV1_stationary_phase_7hours |
| GSM6026 |
delUSV1_stationary_phase_9hours |
| GSM6027 |
delUSV1_stationary_phase_1day |
| GSM6028 |
DBY8778(wt)_stationary_phase_3hours |
| GSM6029 |
DBY8778(wt)_stationary_phase_5hours |
| GSM6030 |
DBY8778(wt)_stationary_phase_7hours |
| GSM6031 |
DBY8778(wt)_stationary_phase_9hours |
| GSM6032 |
DBY8778(wt)_stationary_phase_1day |
| GSM6033 |
delUSV1_stationary_phase_0hours |
| GSM6034 |
delUSV1_stationary_phase_2hours |
| GSM6035 |
delUSV1_stationary_phase_5hours |
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| Relations |
| BioProject |
PRJNA84957 |
| Supplementary data files not provided |
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